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Soil is a rich habitat of microbes which possess the ability to produce hydrolytic enzymes and antimicrobial compounds. Among soil microorganisms, genus Bacillus is well known for production of industrial enzymes, antibiotics and pesticides. This research was aimed to explore amylolytic and antibacterial activity of Bacillus sp. MTZ-1 from rhizosphere of a Guava plant (Psidium guajava). Amylase production was carried out through Shake flask fermentation experiments. Growth and enzyme activities of the strain were observed from 48 to 192 h by spectrophotometric analysis. Crude enzyme preparations also showed antibacterial potential at different time intervals against pathogenic bacterial strains including Vancomycin-resistant Enterococcus (VRE), K. pneumoniae, S. aureus, S. paratyphi A, S. paratyphi B, S. flexneri, B. subtilis and P. mirabilis. Amylase production was maximum at 168 h (134 U/ml) which was further declined at 192 h (76 U/ml) with increased growth. Bacillus sp. MTZ-1 showed strong amylolytic and antibacterial activity, however, both activities remained independent of each other. Antibacterial potential was maximum against S. paratyphi A and VRE (Vancomycin-resistant Enterococcus). Zone of inhibition was maximum (35 mm) against S. paratyphi A at 48 h with no enzyme activity, however, it was 25 mm against VRE with maximum enzyme activity at 168-192 h. Antibacterial activity was found to be independent of the enzyme activity throughout the experiments.
[Zainab Sajid, Maryam Shafique, Sehar Afshan Naz, Sadia Khalil, Tooba Khan (2015); A snapshot of amylolytic and antibacterial activity of Bacillus sp. MTZ-1 from indigenous soils Int. J. of Adv. Res. 3 (4). 0] (ISSN 2320-5407). www.journalijar.com
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