30Apr 2015

Octadecane biodegradation by Bacillus spp. isolated from contaminated oily soil

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Octadecane is an alkane hydrocarbon with the chemical formula CH3(CH2)16CH3 and the efficiency of Bacillus spp. strains isolated from a petroleum contaminated soil for the biodegradation of octadecane hydrocarbons, is studied after cultivation on MSM and MMSM medium, stirred at 150 rpm, for 27 days, at 30ºC. Results showed that the number of viable count of Bacillus spp. was increasing up to 22 days of incubation at 30ºC, then it decreased gradually and reached to 8 × 108 and 2.8 × 108 cell / ml at period 25 and 27 days of incubation, respectively. Dry biomass was increased and reaching maximal density to 3.16gm / l, about 7.2gm / l of the bioemulsion was produced during the first 27 days and isolate have the ability to degraded 62.11% of the weight of octadecane used in the experiment.


[Shurooq, M. Wsain, Adnan, H. Abbas and Doaa Auday Ali Al-Quraishy (2015); Octadecane biodegradation by Bacillus spp. isolated from contaminated oily soil Int. J. of Adv. Res. 3 (Apr). 0] (ISSN 2320-5407). www.journalijar.com


Shurooq, M. Wsain, Adnan, H. Abbas and Doaa Auday Ali Al-Qur