Phylogenetic analysis of heavy metal resistant Streptomyces spp. isolated from soil in Mosul / Iraq
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Biosurfactants are heterogeneous surface active compounds and can be potentially deployed for bioremediation hydrocarbons. In present study 7 strains of Streptomyces spp. from 21 hydrocarbons contaminated soil have been isolated and identified through morphological cultural and biochemical tests. Biosurfactant production was confirmed byconventional screening methods including hemolytic, lipase production, modified drop collapsing and oil spreading methods. Antibiotic & heavy metal resistance phenotypes were determined. The results showed that most of strains were resistance to penicillin10 IU, methicillin 5 µg, erythromycin 10 µg, ceftriaxone 30 µg, tetracycline 30 µg, ampicillin 10 µg, cephalothin 30µg, trimethoprim and sulfaminoxazole 25 µg.The isolates showed resistant to 1000µg/ml of Nickel sulphate , Zinc sulphate , Lead acetate& nitrate, Silver acetate& chloride, Titanium dioxide , Cobalt nitrate & chloride. Also all isolates showed sensitive to Mercuric sulphate & chloride. The results of polymerase chain reaction (PCR)showed that bands of 16s rDNA using universal primers 27f & 1392r were found at 1350 bp compare with the 100bp ladder.The nucleotide sequence of the 16s rDNA gene identified two strains that similar to Strepto.flavogriseusATCC 33331 &five strains belongs toStrepto.albusJ1074. Thefive strains belongs toStrepto.albusJ1074 grouped in cluster A at similarityof 97.5%& the two strains that belongs to Strepto.flavogriseusATCC 33331 accumulate in cluster B at similarityof 99%. The phylogenetic tree depending on the nucleotide sequence using Clustal W program and the UPGMA method.The evolutionary distances were computed using the Maximum Composite Likelihood method based on the Tamura model distance coefficient with neighbor joining (NJ), using Mega 0.5.
[Essra Gh. Alsammak (2014); Phylogenetic analysis of heavy metal resistant Streptomyces spp. isolated from soil in Mosul / Iraq Int. J. of Adv. Res. 2 (2). 0] (ISSN 2320-5407). www.journalijar.com
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