INHIBITION OF ADJUVANT INDUCED POLY ARTHRITIS IN RATS BY VARIOUS BARK EXTRACT/FRACTIONS OF SYMPLOCOS RACEMOSA

1. Department of Pharmacy, Babu Banarsi Das National Institute of Thecnology & Management, Lucknow, India. 2. Department of Pharmacy, Chitkara University, Rajpura, India. 3. Department of Biotechnology, Integral University, Lucknow, India. 4. Faculty of Pharmacy, Hamdard University, Delhi, India. ...................................................................................................................... Manuscript Info Abstract ......................... ........................................................................ Manuscript History

Ash contains carbonate of soda and large quantity of coloring agents (10). It also inhibit the enteric and dysenteric group of microbes and to reduce the frequency and intensity of the contractions in vitro of both pregnant non pregnant uteri (11).

Materials and Method:-Plant collection and extraction:-
The barks of Symplocos racemosa were collected from herbal garden of BBDNITM, Lucknow, India and were identified in N.B.R.I., Lucknow, India. The dried bark powder was extracted with various solvents and solvents were concentrated separately and dried. The dried extracts were preserved until further use.

Animals:-
Sprague Dawley rats (120-150 g) were used in the experiments. All animals were fed with a standard diet ad libitum and have free access to drinking water. Animal study was performed in institutional animal house according to CPCSEA norms.

Inhibition of adjuvant induced poly arthritis in Rats:-
The separated fractions from the plant were screened for their capacity to inhibit adjuvant induced poly arthritis in rats at different doses given intraperitonealy (Administered daily from days 15-22).

Statistical Analysis:-
The data is presented as mean ± SEM. The data was analyzed by one way ANOVA. Different value of P at different days interval were significant. Group I-Arthritic rats treated with saline; Group II -Arthritic rats treated with SRAF 20 mg/kg; Group III -Arthritic rats treated with SRAF 100 mg/kg; Group IV -Arthritic rats treated with SRSF-1 20 mg/kg; Group V -Arthritic rats treated with SRSF-1 100 mg/kg; Group VI -Arthritic rats treated with SRSF-2 20 mg/kg; Group VII -Arthritic rats treated with SRSF-2 100 mg/kg;Group VIII Arthritic rats treated with Indomethacin; *p < 0.05, @p < 0.01, #P <0.001as compared to arthritic control. SRAF showed inhibition of adjuvant edema earlier on from 16 th day onwards both for higher nontoxic dose and from day 18 th at lower dose of 20 mg/kg and produced more significant inhibition as compared to the lower dose 20 mg/kg (p<0.05 from 19 th day, p<0.01 from 22 nd day) and higher nontoxic dose (p<0.05 from 18th day, p<0.01 from 21 st day) for the SRSF-1 fraction. SRSF-2 fraction was similar in response to SRAF and showed statistically significant inhibition of arthritic lesions (p<0.05) from day 16, (p<0.01) from day 20 and (p<0.001) from day 21 onwards.