27Feb 2017
GREEN SYNTHESIS, CYTOTOXICITY AND UTILIZATION OF CARBOXYMETHYLCHITOSAN-STABILIZED GOLD NANOPARTICLES.
- Pre-Treatment & Finishing of Cellulosic Fibers, Textile Research Division, National Research Centre, 33 El Bohouthst. (Former El Tahrir St.), Dokki, Giza, Egypt, P.O.12622.
- Physics Department, Faculty of Girls for Arts, Science and Education, Ain Shams University, Cairo, Egypt.
- Faculty of Applied Arts, Printing, Dyeing and Finishing Department, Benha University, Benha, Egypt.
- Faculty of scienceEjelat, University of Zawia, Libya.
- Abstract
- References
- Cite This Article as
- Corresponding Author
Gold nanoparticles (AuNPs) was prepared via simple and green method by using polysaccharides as reducing and stabilizing agents at the same time. Hiren we used carboxymethylchitosan (CMCS) as a reducing agent for gold nanoparticle as well as capping agent. CMCS prepared based on our previous method by reacting chitosan with monochloroacetic acid in alkaline medium. AuNPs were prepared by using different concentrations of carboxymethylchitosan (0.2% w/v, 0.5% w/v and 1% w/v) at 100 oC for 1 hour. CMCS was characterized by using nitrogen content, carboxyl content and FTIR spectra. AuNPswas characterized by using UV spectrophotometry and TEM images. Finally, the cytotoxicity of the prepared AuNPs were evaluated using cell viability assay from MMT and IC50 values compared with AuNPs prepared by chemical methods. The results shows that AuNPs have normal distributed with 15-25 nm particle size and its cytotoxicity was lowered when prepared by this green method and can use GNPs safely in contact medical treatment with skin.
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[H. M. Ibrahim, A. Dakrory, T. M. Tawfik and H. A. Zgeiup. (2017); GREEN SYNTHESIS, CYTOTOXICITY AND UTILIZATION OF CARBOXYMETHYLCHITOSAN-STABILIZED GOLD NANOPARTICLES. Int. J. of Adv. Res. 5 (Feb). 1588-1596] (ISSN 2320-5407). www.journalijar.com
Hassan M. Ibrahim
National Research Center
National Research Center
