Effect of Gold Nanoparticles on Gram negative bacteria Aeromonas hydrophila

In order to know the effect of the gold nanoparticles on Aeromonas hydrophila antigens on the white mice , ( 18 ) mice ( from both sex ) was used and divided onto 5 groups each group immunized with 0.2 ml of the extracted antigens except the control group which injected with 0.2 ml of phosphate buffer saline . serum collected after ( 20 ) days of immunization. Several immunological methods was used to determine the presence of antibodies that formed as a result of the mouse immune response . The results showed that most of the used antigens in the study gives positive results in SAT test while the LPS antigens with 10 mg/ml and Pili antigen gives negative gives negative results to the presence of antibodies on slide agglutination test . While all antigens understudy gives appositive results to the presence of Antibodies in ELISA Test , and This results was confirmed by the using of counterimmunoelectrophoresis technique which give the same result with ELISA test .The immunization with gold nanoparticles showed higher immune responses for the antibodies . it was noticed from the immunological test results that the antigens with the gold nanoparticles gives high percentage for the presence of antibodies which indicate that these antigens made strong immune response in comparison with the non-gold nanoparticles antigens. Also the LPS antigens with 200 mg/ml and the whole cell antigens of A. hydrophila gives the highest percentage for the presence of antibodies in comparison with the other extracted antigens used understudy. This results was compared with control samples which give negative result to the present of antibodies.