VARIATIONS INDUCED IN ELECTROPHORETIC PATTERN OF HAEMOLYMPH PROTEINS OF FLESH FLY, SARCOPHAGA ARGYROSTOMA (DIPTERA: SARCOPHAGIDAE) LARVAE CHALLENGED WITH HYDROGEN PEROXIDE.
- Zoology Department, Faculty of Science, Menoufia University, Menoufia, Egypt.
- Zoology Department, Faculty of Science, Al-Azhar University (Girls branch), Nasr city, Cairo, Egypt.
- Entomology Department, Faculty of Science, Cairo University, Giza, Egypt.
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Insects like all aerobic organisms are continuously exposed to oxidative stress due to products produced during oxygen metabolism and via the metabolism of the encountered toxins including allelochemicals and pesticides. This study was conducted to determine the variation induced in electrophoretic pattern of haemolymph proteins of third instar larvae of flesh fly, Sarcophaga argyrostoma challenged with hydrogen peroxide. Haemolymph was collected every four hours interval for seventy two hours post-injection with hydrogen peroxide. Total soluble protein of the haemolymph was extracted and separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Hydrogen peroxide challenge induced differences in number of electrophoretic protein bands with different molecular weights as compared to control. These results clearly showed that larval challenge with hydrogen peroxide could evoke the haemolymph to synthesize new proteins to overcome such stress. The quantitative analysis also clearly indicated variations in the number as well as intensity of the protein bands.
[S.H. Mahmoud, W.A. Moselhy, L.A.A. El-Khashab2, B. Z. Abdelbaset and A.M. Seufi. (2016); VARIATIONS INDUCED IN ELECTROPHORETIC PATTERN OF HAEMOLYMPH PROTEINS OF FLESH FLY, SARCOPHAGA ARGYROSTOMA (DIPTERA: SARCOPHAGIDAE) LARVAE CHALLENGED WITH HYDROGEN PEROXIDE. Int. J. of Adv. Res. 4 (Oct). 33-43] (ISSN 2320-5407). www.journalijar.com
