ASSESSMENT OF IUI OUTCOME FOR INFERTILE COUPLES WITH MALE INFERTILITY USING SPERM MAX PURE VERSUS DENSITY GRADIENT TECHNIQUES.

Background:Different techniques are used for separation of motile and morphologically normal spermatozoa to be used for artificial insemination (AI) and other assisted reproductive techniques.Sperm preparation is important because some component of seminal fluid may become obstacle to fertilization when IUI or IVF are performed. However the choice of sperm selection technique depend on the quality of the semen in order to obtain the higher number of normal spermatozoa. Objective: To assess the pregnancy rate for infertile couples undergoing IUI using Maxpure technique versus density gradient technique to prepare sperm from males with male infertility. Subjects, Materials and Methods: One hundred twenty seven (127) infertile couples participated in this study during their attendance to the infertility clinics at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. The period of study was from September 2015 to June 2016. The mean age for all males included in this study was 35.33±0.731 and the mean age for all females included in the study was 30.67±0.711 years. The mean duration of infertility was 6.18±0.403 ranged from 1- 21 years. Semen analysis was done on the collected semen samples according to WHO (2010). From each semen sample two mL was taken. One mL was prepared using density gradient centrifugation technique, and the other one mL prepared using sperm Maxpure technique. Then sperm parameters were assessed for these two techniques, and the part with the best result had been used for intrauterine insemination and finally the pregnancy results were statistically analyzed. Results: The reduction in sperm concentration was significantly (P<0.001) far more using Density gradients technique, mean progressive motile sperm (%) was significantly (P<0.001) more raised using sperm Max pure technique. However non-significant change (P=0.430)was observed regarding mean non progressive motile sperm (%). Mean immotile sperm (%) was far less using sperm Max pure technique (P<0.001).In contrast mean normal sperm morphology (%) was significantly higher using sperm Max pure technique (P<0.001). Finally, the round cell count/HPF was significantly (P<0.001) less using sperm Max pure technique.