01Jan 2017

Phytochemical screening and Antimicrobial activity of Euphorbia linearifolia var.nallamalayana J.L.Ellis from the Nucleus of Eastern Ghats.

  • Department of Botany and Microbiology, Nagarjuna University, Guntur, Andhra Pradesh, India. Department of Botany and Microbiology, Acharya Nagarjuna University Guntur, Andhra Pradesh, India.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as

The current study was focused to investigate the phytochemical analysis, antibacterial and antifungal activity of plant extract of Euphorbia nallamalayana against different pathogens. Four solvent extracts were evaluated for antimicrobial activity using well diffusion method and MIC determination on different micro organisms. The shade dried plant material was subjected to different solvents for phytochemical screening. The antibacterial activity was done using well diffusion method against eight potentially pathogenic microorganisms Aspergillus niger,Aspergillus flavus,Candida albicans, Saccharomyces cerevisiae , Escherchia coli , Staphylococcus aureus, Bacillus subtilus and Pseudomonas aeruginosa. Phytochemical screening was done using standard qualitative methods which revealed the presence of Tannins, Saponins, Steroids, coumarins, Flavones, Flavanones, Phenols, Quinones and Carbohydrates. There was no antifungal activity with Asperigillus flavus,Candida albicans and Saccharomyces cerevisiae.The findings of this study indicate that the plant extract of Euphorbia nallamalayana showed significant antibacterial activity against both gram positive and gram negative organisms. We also found the antifungal activity against Aspergillus niger. However, further studies on isolation characterization of plant extract responsible for antibacterial activity is necessary to elucidate their mechanism of action and it would give comprehensive evidence of bioactive potential of medicinal plants at cellular and molecular levels. Euphorbia nallamalayana has a strong antimicrobial potential, which justifies its use in folk medicine for treatment of infectious diseases. Since this species is poorly investigated, further refined studies on it pure secondary metabolites are needed and very important, in the perspective to identify new antimicrobial molecules from this endemic plant.


  1. Zaika L.L. (1975): Species and herbs their antimicrobial activity 9:97-118.
  2. Rastogi RP, Mehrotra BN (2002): Glossary of Indian medicinal plants. National Institute of Science Comunication, New Delhi, India.
  3. Gon?alves S, Gomes D, Costa P, Romano A. (2013): The phenolic content and antioxidant activity of infusions from Mediterranean medicinal Ind Crops Prod; 43(1): 465-471.
  4. Luo H, Li Q, Flower A, Lewith G, Liu J. (2012): Comparison of effectiveness and safety between granules and decoction of Chinese herbal medicine: A systematic review of randomized clinical trials. J Ethno pharmacol 140(3): 555-567.
  5. Radulovic NS, Blagojevic PD, Stojanovic-Radic ZZ, Stojanovic (2013): Antimicrobial plant metabolites: Structural diversity and mechanism of action. Curr Med Chem 20(7): 932-952.
  6. Morens DM, Folkers GK, Fauci AS. (2008): Emerging infections: a perpetual challenge. Lancet Infect Dis 8(11): 710-719.
  7. R?os JL, Recio MC. ( 2005): Medicinal plants and antimicrobial activity. J Ethno pharmacol 100(1-2): 80-84.
  8. Grill MF, Maganti RK. (2011): Neurotoxic effects associated with antibiotic use: management considerations. Br J Clin ?Pharmacol ?72(3): 381-393.
  9. Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Riaz N, (2010): Antimicrobial natural products: an update on future antibiotic drug candidates. Nat Prod Rep 27(2): 238-254.
  10. Hooper DC, DeMaria A, Limbago BM, O?Brien TF, McCaughey B. (2012):Antibiotic resistance: how serious is the problem, and what can be done? Clin Chem ?58(8): 1182-1186.
  11. Hemaiswarya S, Kruthiventi AK, Doble M. (2008): Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 15(8): 639-652.
  12. Gordon M.C and David J.N. (2001):Natural product drug discovery in the next millennium. Pharm Biol. 139:8-17
  13. Vedavathy S, Mrudula V and Sudhakar A Tribal in Chitoor District, Andhra Pradesh, India. Vedams e book P,Ltd 1977.
  14. Pei,S.J.(2001): Ethanobotanical approaches of traditional medicine studies: Some experiences from Asia. Pharmaceutical biology 39:74-79.
  15. Fabricant D.S and Fansworth N.R. (2001): The value of plant used in traditional medicine for drug discovery. Environ. Health Perspect. 109:69-79.
  16. Al-Bayati F.A and Al-Mola H.F. (2008): Antibacterial and antifungal activity of different parts of Tribulus terrestris L growing in Iraq: J. Zhejiang Univ. Sci. B. 9:154-159.
  17. Evans W.C. Trease and Evans (1996): pharmacognosy 14th WB Sacender Company Ltd., pp.290.
  18. Fabricant D.S and Fansworth N.R. (2001): The value of plants used in traditional medicine for drug discovery. Environ. Health Perspect. 109;69-79.
  19. http://www.theplantlist.org/tpl1.1/record/kew-80596.
  20. Ahmed,Z.U.Begum,Z.N.T.,Hassan,M.a.Khonder,M.,Kabir,S.M.H.,Ahmad,M.,Ahmed,A.T.A.Rahman,A.K.A,Haque,E.U.(Eds). Encyclopedia of Flora and Fauna of Bangladesh Dicotyledons. Asiat. Soc. Bangladesh, Dhaka, 2007-2009,Vol 6-12,
  21. Charles, C. D., Maribeth, L., Daniel, L. N., Kenneth, J. W., David, A. B. Floral Gigantism in Rafflesiaceae Science Express ,USA.2007.
  22. Ravindra, B., Malabadi, Gangadhar, S., Mulgund and Nataraja, K. (2005):Screening an antibacterial activity in the extracts of Clitorea ternatea , Jour medical and Aromatic Plant Sciences, 27,26-29.
  23. Prusti,A., Misra,S.R.,Sahoo,S. and Mishra,S.K., (2008):Antibacterial activity of some Indian medicinal plants, Ethnobotanical leaflets, 12,227-230.
  24. Nair, R., Kalariya, T., Sumitra chanda., (2005): Antibacterial activity of some selected Indian medicinal flora. Turk J Biol, 29,41-47.
  25. Ahmad, I., Mehmood, Z and Mehmood, I., (1998): Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol, 62,183-193.
  26. Arjuna Patra , Jha,S., Murthy,P.N. Satpathy, S., Antibacterial activity of Hygrophila Spinosa T.Andres (2009): leaves. A comparative study. Int J Pharm Tech Res, 1(3), 837-839.
  27. Pullaiah T & Ali. Moulali D. (1977): Flora of Andhra Pradesh ,1st ed ,Vol ? II,Jodhpur ;Scientific Publishers.
  28. Ellis JL. (1987): Flora of Nallamalais ,Vol -II Botanical Survey of India.
  29. Anonymous, (1996) The Indian pharmacopoeia Government of India, New Delhi. Ministry of Health and family welfare;.
  30. National Committee for Clinical Laboratory Standards. Performance Standards for Anti-Microbial Susceptibility Testing: Eleventh International Suppliment. NCCLS,2001, M100-S11.
  31. Amarsingham P.P, Bisset, G, Millard P.H, Woods M.C, (1964): Phytochemical Survey of Malaya part 3.Alkaloids and Saponins. Economic Taxonomic Botany 18:270-278.
  32. Sahm DF, Washington JA (1990). Antibacterial susceptibility tests: Dilution methods. In: Manual of clinical Microbiology. Lennette, EH (ed.), 5th edition. American Society of Microbiology, Washington, DC. pp. 1105-1118.
  33. Harborne, J.B,. Phytochemical methods: A guide to modern techniques of plant analysis Champman & Hall , London 973,279.
  34. Raaman N. (2006) Phytochemical Techniques . New India Publishing Agency.New Delhi.
  35. Rahman, A., Parvez, M.A.K., Parvin, T., Paul, D.K., Sattar, M.A., (2004): Antimicrobial activity of three bioactive compounds from the stream bark of Piper chaba hunter. Bioscie. Res., 1: 16 20.
  36. Bhattacharjee, I., Chatterjee, S.K., Chatterjee, S., Chandra, G. (2006): Antibacterial potentiality of Argemone mexicana solvent extracts against some pathogenic bacteria. Memorias do Instituto Oswaldo Cruz., 101: 645 648.
  37. Reynolds, J.E.F. Martindale (1996): the extra harmacopoeia, 31st edn, Royal Pharmaceutical Society of Great Britain, London.
  38. Bezic, N.M., Dinkic, S.V., Radonic, (2003): A., Composition and antimicrobial activity of Achillea clavennae essential oil. Phytother. Res., 17: 1037 1040.
  39. Rahman, M.M., Alam, J., Sharmin, S.A., Rahman, A., Alam, M.F. (2009): In Vitro antibacterial activity of Argemone mexicana (Papaveraceae). CMU J. Nat. Sci., 8: 77 84.
  40. Joshi, B., Sah, G.P., Basnet, B.B., Bhatt, M., Sharma, D., Subedi, K., Pandey, J., Malla, R., (2011): Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). J. Microbiol. Antimicrob., 3(1): 17.
  41. Arulmozhi, S., Mazumder, P.M., Ashok, P., Narayanan, L.S., (2007):Pharmacological activities of Alstonia scholaris (Apocynaceae)- A review. Pharmaco. Rev., 1: 163 165.

[D.Nirmala Grace and Z.Vishnu Vardhan. (2017); Phytochemical screening and Antimicrobial activity of Euphorbia linearifolia var.nallamalayana J.L.Ellis from the Nucleus of Eastern Ghats. Int. J. of Adv. Res. 5 (1). 326-333] (ISSN 2320-5407). www.journalijar.com


DOI:


Article DOI: 10.21474/IJAR01/2750       DOI URL: http://dx.doi.org/10.21474/IJAR01/2750


Share this article