20Jan 2017

ANTIBACTERIAL ACTIVITY OF MICROORGANISMS ASSOCIATED WITH MARINE INVERTEBRATES FROM THE MOROCCAN ATLANTIC COAST.

  • Laboratoire Controle Qualite en Bioindustrie et Molecules Bioactives, Faculte des Sciences, Universite Chouaib Doukkali, BP 20, 24000 El-Jadida, Maroc.
  • Laboratoire du Maghreb, BP 2863, Rabat, Maroc.
  • Laboratoire Biomolecules et Synth?se Organique, Facult? des Sciences Ben M?sik, Universite Hassan II-Casablanca, B.P 7955 Casablanca, Maroc.
  • Ecole nationale de commerce et de gestion, BP 122 El-Jadida, Maroc.
  • Physicochimie des Milieux Naturels et Mol?cules Bioactives (PMNMBA), Facult? Polydisciplinaire de Taroudant, Universit? Ibn Zohr, BP 271, 83000 Taroudant, Maroc.
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In vitro antibacterial screening of bacterial strains isolated from marine invertebrates, collected from the Moroccan Atlantic coast, against human pathogenic bacteria was conducted in this study. However fourteen marine microorganisms were tested against three Gram-positive bacteria (Staphylococcus aureus, Bacillus sp. and Enterococcus faecalis) and three Gram negative bacteria (Escherichia coli, Pseudomonas fluorescens and Pseudomonas sp.) using the agar disk-diffusion and the agar cylinders methods. The identification of the isolates shows that are belong to the genera Enterobacter, Morganella, Aeromonas, Pantoea, Kluyvera, Raoultella, Stenotrophomonas, Pseudomonas, Sphingomonas and Staphylococcus which indicates a diversification in the marine microflora. The evaluation of antibacterial activity of these isolates on human pathogenic bacteria shows that all strains are active against at least one pathogen studied for the two used methods. For the agar cylinders method, six isolates are showing a significant antibacterial activity with inhibition zone diameters greater or equal to 15 mm (Aeromonas sobria, Enterobacter cloacae, Stenotrophomonas maltophilia, Staphylococcus capitis, Pantoea sp3 and Raoultella ornithinolytica). For the agar disk-diffusion method, among 28 extracts of the marine isolates, five extracts which are active against two pathogenic bacteria (Pseudomonas sp and Enterococcus faecalis) with inhibition zone diameters greater or equal to 15 mm (two ethanol extract of Enterobacter cloacae , ethanol extract of Raoultella ornithinolytica, ethanol extract of Morganella morganii and ethanol extract of Aeromonas sobria). By comparing the two used methods we can conclude that the agar cylinders method gave better results compared to the agar disk-diffusion method.

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[Mohammed El Amraoui, Meriem Tarbaoui, Majida El Wahidi, Aziz Fassouane, Belkassem El Amraoui and Toufiq Bamhaoud. (2017); ANTIBACTERIAL ACTIVITY OF MICROORGANISMS ASSOCIATED WITH MARINE INVERTEBRATES FROM THE MOROCCAN ATLANTIC COAST. Int. J. of Adv. Res. 5 (Jan). 1127-1133] (ISSN 2320-5407). www.journalijar.com

Meriem TARBAOUI
Laboratoire Biomolécules et Synthèse Organique, Faculté des Sciences Ben M’sik, Université Hassan II-Casablanca, B.P 7955 Casablanca, Maroc

DOI:

Article DOI: 10.21474/IJAR01/2862      
DOI URL: http://dx.doi.org/10.21474/IJAR01/2862

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