20Mar 2017

IN VITRO ANTI INFLAMMATORY AND ANTI MICROBIAL PROPERTIES OF ALLOPHYLUS SERRATUS LEAF EXTRACTS.

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Allophylus serratus is a local medicinal plant used traditionally for the treatment of diarrhea, inflammation, ulcer, elephantiasis and gastro intestinal disorders. The leaf extracts of this plant were assessed for in vitro anti-inflammatory and antibacterial activities. The in vitro anti inflammatory activity assay of leaf extracts of Allophylus serratus were evaluated by Human Red Blood Cells membrane stabilization, inhibition of protein denaturation and protease inhibition methods. The anti microbial activity of leaf extracts were evaluated by agar well diffusion method against four bacterial and two fungi species. The membrane stabilization anti inflammatory test showed that, the methanolic leaf extracts exhibited highest membrane stabilizing activity of 77.64?0.78 and 74.77?0.66, compared to that of standard Diclofenac sodium which exhibited 92.65?0.56 % and 89.73?1.18 at 1000 ?g/ml and 800 ?g/ml concentrations respectively. In inhibition of protein denaturation, 400 ?g/ml methanolic extract showed maximum protection 70.73% and standard drug provided 93.50 % protection. But at 1000 ?g/ml the percent inhibition of protein denaturation increased to 95.93%. More over protease inhibition test showed maximum inhibition (67.63%) at 100 ?g/ml concentrations by ethyl acetate extract. In anti inflammatory test, all extraction showed concentration dependent response. In anti microbial activity test the water, methanolic and ethyl acetate extracts were more effective against Escherichia coli and Staphylococcus aureus. Ethyl acetate extract also showed high activity against Bacillus sabtilis (20?0.00mm). Chloroform and petroleum ether extracts showed high zone of inhibition (20?0.17 and 22?0.09 mm respectively) against Pseudomonas aeruginosa but no activity against the rest tested bacterial strains. All the extracts did not show any activity against tested fungal species. The results of this study therefore revealed that leaf extracts of Allophylus serratus possess anti inflammatory and anti bacterial activity which justify the use of this plant in the treatment of bacterial infections and inflammation.

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[Kero Jemal, B.V Sandeep and Sudhakar Pola. (2017); IN VITRO ANTI INFLAMMATORY AND ANTI MICROBIAL PROPERTIES OF ALLOPHYLUS SERRATUS LEAF EXTRACTS. Int. J. of Adv. Res. 5 (Mar). 71-80] (ISSN 2320-5407). www.journalijar.com

Kero Jemal
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Article DOI: 10.21474/IJAR01/3487      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3487

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