20Mar 2017

EFFICIENCY OF SYSTEMIC VERSUS INTRALESIONAL INJECTION OF BONE MARROW CELLS ON BUCCAL WOUND HEALING IN DIABETIC RATS.

  • Teaching assistant, Faculty of Dentistry, Sirte University, Libya.
  • Lecturer of oral biology Mansoura University, Mansoura, Egypt.
  • Professor of oral biology, Mansoura University, Mansoura, Egypt.
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The multipotent capability of bone marrow cells gave an impelling reason to study the effect of bone marrow cells in diabetic wound healing. Diabetes is characterized by delayed and poor wound healing. The goal of this study was to evaluate the efficiency of systemic versus intralesional injection of bone marrow cells on buccal wound healing in diabetic rats. Sixty four adult male albino rats were used in the study and divided randomly into four groups. All groups were subjected to buccal wound induction. Group I; non-diabetic group (negative control) and group II; diabetic group (positive control) and did not receive any treatment. The groups III and IV diabetic groups received intralesional and intravenous injection of whole bone marrow cells respectively two days following wound induction. Specimens were obtained after 3, 7 and 12days following wound induction and were processed to be immunostained with mouse monoclonal antibody PCNA. Immunohistochemical results revealed marked expression of PCNA in treated groups III and IV at day 3 compared to non-treated groups I and II, although there was no significant difference between the treated groups. Our results reported that intralesional injection of whole bone marrow cells showed faster healing than intravenous injection and display a more mature histology. In conclusion, Injection of the whole BMCs intralesional is more efficient compared to intravenous route in accelerating the diabetic oral wound closure and enhancing the wound healing quality. The use of whole bone marrow cells appears to be beneficial in the treatment of diabetic oral wounds.

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[Fatma Ibrahim Elfaiedi, Mona E. Denewar and Fatma M. M. Ibrahim. (2017); EFFICIENCY OF SYSTEMIC VERSUS INTRALESIONAL INJECTION OF BONE MARROW CELLS ON BUCCAL WOUND HEALING IN DIABETIC RATS. Int. J. of Adv. Res. 5 (Mar). 202-212] (ISSN 2320-5407). www.journalijar.com

Mona El Sayed Denewar
Dr.

DOI:

Article DOI: 10.21474/IJAR01/3504      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3504

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