18Jun 2017

PACLOBUTRAZOL MEDIATED ENHANCED MULTIPLICATION OF MUSA PARADISIACA LINN. CV. POOVAN (AAB).

  • Tissue Culture Unit, Plant Genetic Resource Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerala, India.
  • Department of Plant Science, Manonmaniam Sundaranar University, Tirunelveli, 627 012 Tamil Nadu, India.
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An efficient micropropagation system using shoot tip has been developed for Musa paradisiaca cv. Poovan. Among the growth regulators tested, N6-Benzyl adenine (BA) was more effective for inducing multiple shoots. Basal medium supplemented with 5mg L-1BA showed 7shoots/explant with an average shoot growth of 4.1 cm in 20 days. Effect of paclobutrazol was investigated to study its influence on shoot growth and multiplication in ?Poovan?.Paclobutrazol (3 mg L-1) along with BA (5 mg L-1) reduced maximum longitudinal growth of shoots to average 1.62 cm. However, 5 mg L-1BA and 1 mg L-1 paclobutrazol treated cultures achieved substantial growth reduction (2.14 cm) and enhanced the rate of multiplication to 8.2 shoots/ explant in 20 days. The shoots gained normal growth when subcultured onto paclobutrazol free medium; hence it is a temporary phenomenon. The shoots rooted in ? MS + 150 mg L-1 activated charcoal + 30 g L-1 sucrose + 0.7 mg L-1 IBA and they established successfully in the greenhouse condition with 96 % survival. The enhanced production as well as longitudinal growth reduction with the help of PBZ is beneficial for cost effective micropropagation.

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[Anjana R.G. Nair, P. Ravichandran and M. Bejoy. (2017); PACLOBUTRAZOL MEDIATED ENHANCED MULTIPLICATION OF MUSA PARADISIACA LINN. CV. POOVAN (AAB). Int. J. of Adv. Res. 5 (Jun). 656-663] (ISSN 2320-5407). www.journalijar.com

M. Bejoy
Tissue Culture Unit, Plant Genetic Resource Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695562, Kerala, India; Tel: 0472-2869226; Fax: 0472-2869646; Mobile: 09447450368; Email: mbe_joy@yahoo.com

DOI:

Article DOI: 10.21474/IJAR01/4462      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4462

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