17Jul 2017

PREVALENCE OF AI-1 TYPE OF QUORUM SENSING MOLECULES IN KLEBSIELLA PNEUMONIAE CLINICAL ISOLATES.

  • Department of Microbiology, Panjab University, Chandigarh, India.
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Klebsiella pneumoniae is one of the most frequently isolated bacterial pathogen from cases of nosocomial and community acquired infections. In the present study, 50 clinical strains (Burn wound and urological isolates) along with two standard strains of K. pneumoniae were screened for the presence of recognised quorum sensing system. Although all the isolates were found to be positive for AHL production but the burn wound isolates showed higher AHL production. The clinical isolates showed variable type of signal production therefore bioassay guided expression of quorum sensing signal molecules (AI-1) was employed for qualitative estimation of C12-HSL, C8-HSL, C6-HSL and C4-HSL production by employing A. tumefaciens A-136 (pDSK519) E. coli MG4 (pKDT17), E. coli MG4 (λ1.4), P. aeruginosa PAO-JP2 (pECP61.5), C. violaceum CV026 as their reporters respectively. 100% of the strains of K. pneumoniae were found to produce AHLs, the quorum sensing signal molecule which was subsequently confirmed by HPLC. These results confirm that K. pneumoniae is an avid producer of AI-1 quorum sensing signal molecule.

  1. Arrand S. K., Qin Y., Oger P., (2002) Quorum-sensing system of Agrobacterium plasmids: analysis and utility. Methods Enzymol ; 358 : 452-84.
  2. Balestrino D., Haagensen J. A., Rich C., Forestier C. (2005) Characterization of type 2 quorum sensing in Klebsiella pneumoniae and relationship with biofilm formation. Bacteriol. 187 : 2870-80.
  3. Bjarnsholt T., Jensen P. O., Burmolle M., Hentzer M., Haagensen J., Hougen H., Calum H., Madsen K., Moser C., Molin S., Hoiby N. and Givskov M., (2005). Pseudomonas aeruginos tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum sensing dependent. Microbiology 151 : 373?83.
  4. Cha C., Gao P., Chen Y. C., Shaw P. D., Farrand S. K., (1998) Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant associated bacteria. Mol Plant Microbe Interact 11 : 1119-29
  5. De Araujo C., Balestrino D., Roth, L., Charbonnel N., Forestier C., (2010) Quorum sensing affects biofilm formation through lipopolysaccharde synthesis in Klebsiella pneumoniae. Microbiol. 161 : 595?603.
  6. Fuqua, C., and Eberhard, A., (1999). In:?Cell-Cell Communication in Bacteria. American Microbiology Association Press; 1999.
  7. Khajanchi B. K., Kirtley M. L., Brackman, S. M. and Chopra, A. K. (2011) Immunomodulatory and Protective Roles of Quorum-Sensing Signaling Molecules N-Acyl Homoserine Lactones during Infection of Mice with Aeromonas hydrophila. Infection and Immunity 79 : 2646?2657.
  8. Marketon M. M., Gronquist M. R., Eberhard A. and Gonzalez J. E.,(2002) Characterization of the?Sinorhizobium meliloti sinR/sinI?Locus and the Production of Novel?N-Acyl Homoserine Lactones. Journal of Bacteriology 184 : 5686-95.
  9. McClean K. H., Winson M. K., Fish L., Taylor A., Chhabra S. R., Kamara M (1997) Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143 : 3703-11.
  10. Passador L., Cook, J. M., Gambello M. J., Rust, L. and Iglewski B. H. (1993). Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science, 260 : 1127-30.
  11. Pearson J. P.,?Passador L.,?Iglewski B. H.,?and Greenberg, E. P. (1995) A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa. Proc Natl Acad Sci U S A.28: 92 (5) :1490-4.
  12. Stickler D. J., Morris, N. S., Mclean R, J., Fuqua C. (1998) Biofilms on indwelling urethral catheters produce ruorum-sensing signal molecules in situ and in vitro. Appli Environ Microbiol 64 : 3486-71.
  13. Thiel V., Kunze B., Verma P., Wagner-Dobler I. and Schulz, S. (2009). New structural variants of homoserine lactones in bacteria. A European Journal of Chemical Biology 10 : 1861-68.
  14. Yin F.N., Cheng H. J., Chan J. W., Yin W.F., Chan K.G. (2013) Short Chain N-Acylhomoserine Lactone Production by Clinical Multidrug Resistant Klebsiella pneumoniae Strain CSG20. Sensors 13 : 15242?251.
  15. Yin W.F., Purmal K., Chan S., Chan X.Y., Chan, K.G. (2012) Long chain N-acyl homoserine lactone production by enterobacter sp. isolated from human tongue surfaces. Sensors 12 : 14307?314.
  16. Zhu H. et al., (2002) Determination of quorum-sensing signal molecules and virulence factors of Pseudomonas aeruginosa isolates from contact lens-induced microbial keratitis. J Med Microbiol 51 : 1063-70.
  17. Zhu H., Liu H. J., Ning S. J. and Gao Y. L. (2012) The Response of Type 2 Quorum Sensing in Klebsiella pneumoniae to a Fluctuating Culture Environment. DNA and Cell Biology 31 : 455-59.

[Kapil Mukesh, Kusum Harjai and Sanjay Chhibber. (2017); PREVALENCE OF AI-1 TYPE OF QUORUM SENSING MOLECULES IN KLEBSIELLA PNEUMONIAE CLINICAL ISOLATES. Int. J. of Adv. Res. 5 (Jul). 101-107] (ISSN 2320-5407). www.journalijar.com

Sanjay Chhibber
Panjab University

DOI:

Article DOI: 10.21474/IJAR01/4699      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4699

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