17Jul 2017

SCREENING OF FEATHER DEGRADING BACTERIA AND ITS APPLICATION.

  • Department of Microbiology, St. Xavier?s College-Autonomous, Mumbai-400001, Maharashtra, India.
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Environmental pollution is an issue of global concern and feather waste which is a byproduct of the growing poultry processing industry is an example of a pollutant not easily degraded by common proteolytic enzymes. However, feathers when acted upon by keratinolytic microbes are degraded to smaller peptides and amino acids which can be used as dietary supplements in animal feed. In the present study, feather degrading microorganisms isolated from poultry farm soil were initially screened for proteolytic activity of the organism on skim milk agar plates. The selected organism was subsequently grown on feather meal medium and biochemically, genotypically characterized and identified to be a Bacillus species. Significant keratinase activity was observed over a wide range of pH for six days, using the keratinase assay. The organism showed good bioremediation activity in broth as well as in soil with the crude feather. Further, it was confirmed that the organism can also degrade feathers using table sugar as a cheap carbon source. Optimum conditions for amino acid production were determined by the quantitative ninhydrin method which confirmed significant amino acid production, under both shaker and static conditions, after 96 hours of incubation at room temperature.

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[Ruhi Patrawala, Ria Odakayi, Sankalpa Palkar, Cyd Rodricks, Algamary Benny, Vivien Amonkar and Pampi Chakraborty. (2017); SCREENING OF FEATHER DEGRADING BACTERIA AND ITS APPLICATION. Int. J. of Adv. Res. 5 (Jul). 565-573] (ISSN 2320-5407). www.journalijar.com

Pampi Chakraborty
Department of Microbiology, St. Xavier’s College-Autonomous, Mumbai-400001, Maharashtra, India

DOI:

Article DOI: 10.21474/IJAR01/4753      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4753

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