20Jul 2017

ISOLATION AND MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS SPP. FROM BOVINE MASTITIS MILK SAMPLES.

  • Ashok & Rita Patel Institute of Integrated Study & Research in Biotechnology and Allied Sciences, New Vallabh Vidyanagar-388121, Gujarat, India.
  • Department of Animal Biotechnology, College of Veterinary Science and A. H., Anand Agricultural University, Anand-388001, Gujarat, India.
  • Center for Interdisciplinary Studies in Science and Technology, Sardar Patel University, Vallabh Vidyanagar-388121, Gujarat, India.
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Mastitis is an inflammation of mammary gland caused by major etiological agent Staphylococcus sp. mainly antibiotic resistant strains. In this study, antibiotic resistant Staphylococcus spp. were isolated, characterized and confirmed by molecular techniques from a total of 120 lactating cows including 30 buffaloes (480 quarter milk samples). They were screened for mastitis using Somatic Cell Count (SCC) and California Mastitis Test (CMT). Isolation and identification of Staphylococcus spp. was done using standard microbiological methods by species specific media as well as various biochemical tests. Antibiotic susceptibility tests (ABST) were performed to screen resistant isolates. Genotypic identification was confirmed using 23S rRNA amplification and 16S rDNA sequencing. A total of 193 non-duplicated Staphylococcus spp. were isolated having higher than 500 somatic cells/μl and CMT score was 1 or 2. Methicillin resistance in 31 and other multidrug resistivity in 17 Staphylococcus spp. were observed. Multidrug resistant strains were identified using Sanger sequencing and assigned accession number KX821621 to KX821638 for 11 Staphylococcus aureus and 7 coagulase negative Staphylococcus spp. These findings suggested that milk represents a potential infection risk threat of multidrug-resistant S. aureus in India due to neglected hygienic practices during lactation and maintenance of herd.

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[Khushal Patel, Chaitanya Joshi and Anju Kunjadiya. (2017); ISOLATION AND MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS SPP. FROM BOVINE MASTITIS MILK SAMPLES. Int. J. of Adv. Res. 5 (Jul). 1080-1088] (ISSN 2320-5407). www.journalijar.com

Dr. Anju P. Kunjadiya
Center for Interdisciplinary Studies in Science and Technology, Sardar Patel University, Vallabh Vidyanagar-388121, Gujarat, India

DOI:

Article DOI: 10.21474/IJAR01/4818      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4818

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