17Jul 2017

SCREENING AND EVALUATION OF ANTIBACTERIAL SUBSTANCES FROM BACTERIAL CULTURES OF INDO-GANGETIC PLAINS.

  • Department of Biotechnology, A.N. College, Patna.
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Resistance to Drug in various pathogenic and non pathogenic bacteria has been found to be increasing. To overcome this problem there is a dire need to search for new antibacterial compounds from various sources. With this perspective in mind bacterial isolates from four different sites of Indo-gangetic plain soils were tested. These bacterial cultures were isolated and assayed for their antibacterial compounds produced and tested their efficacy on three test system (Escherichia. coli, Staphylococcus aureus and Klebsiella pneumonia). The sonicated sample of the isolates as well as supernatant was used for this purpose to evaluate antibacterial property. Mass cultures of isolates were centrifuged to get the bacterial cell pellet and supernatant. Out of ten selected bacterial isolates, nine exhibited inhibitory activity against the test organisms. The zone of inhibition generated during this study has been noticed in the range of 0.9 cm to 1.6 cm on Klebsiella pneumonia, 1.2 cm to 1.5 cm on Escherichia coli and 0.95 to 1.3 on Staphylococcus aureus. Therefore, it is suggestive that Indo gangetic soil could be an interesting source to explore bacteria having unique feature of producing antibacterial compounds and this may be utilized for commercial value.

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[Priyanka and Chandrawati Jee. (2017); SCREENING AND EVALUATION OF ANTIBACTERIAL SUBSTANCES FROM BACTERIAL CULTURES OF INDO-GANGETIC PLAINS. Int. J. of Adv. Res. 5 (Jul). 751-756] (ISSN 2320-5407). www.journalijar.com

Priyanka
Department of Biotechnology, A.N. College, Patna.

DOI:

Article DOI: 10.21474/IJAR01/4776      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4776

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