24Jul 2017

IN VITRO MICROPROPAGATION OF STACHYTARPHETA JAMAICENSIS L.VAHL., AN ETHNOMEDICINAL PLANT AND CONFIRMATION OF GENETIC FIDELITY OF THE PLANTLETS USING RAPD MARKERS.

  • Department of Botany, Kakatiya University, Warangal- 506009, Telangana, India.
  • Department of Biotechnology, Kakatiya University, Warangal- 506009, Telangana, India.
  • Molecular Biology Section, Central Sericultural Research &Training Institute, Central Silk Board, Ministry of Textiles, Government of India, Pampore, Jammu & Kashmir, India
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In vitro shoots proliferated from shoot tip explants on MS medium supplemented with various concentrations of BAP, KN & TDZ and rooting of individual shoots occurred on MS medium supplemented with various concentration of IBA, IAA & NAA in Stachytarpheta jamaicensis L. Vahl. Maximum number of shoots & longest shoot length (cm) (1.96  0.17) & (2.34 0.16) respectively, were produced on MS + TDZ (0.5mg/L), after 4 weeks of culture. Maximum number of roots and longest root length (cm) (2.67  0.88) & (3.33  0.33) respectively, were produced on MS + IBA (0.5 mg/L), after 4 weeks of culture. The rooted plantlets (R1) were acclimatized in green house for 4 weeks and then transferred to field conditions with 69% survivability. RAPD primer OPE-20 banding pattern of R1 plantlets was similar to that of mother plant.

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[Korra Rajender, Bylla Prasad, Gulab Khan Rohela, Sreenu Pendli1 and T. Christopher Reuben. (2017); IN VITRO MICROPROPAGATION OF STACHYTARPHETA JAMAICENSIS L.VAHL., AN ETHNOMEDICINAL PLANT AND CONFIRMATION OF GENETIC FIDELITY OF THE PLANTLETS USING RAPD MARKERS. Int. J. of Adv. Res. 5 (Jul). 1494-1502] (ISSN 2320-5407). www.journalijar.com

Dr. T. Christopher Reuben
Kakatiya University

DOI:

Article DOI: 10.21474/IJAR01/4862      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4862

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