10Oct 2017

MOSQUITO LARVICIDAL AND CYTOTOXIC STUDIES ON ANNONA RETICULATA L.

  • Bishop Moore College, Mavelikara 690 110.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Mosquitoes transmit serious human diseases, causing millions of deaths every year. Natural products of plant origin with insecticidal properties have been used in recent years for control of a variety of pest insects and vectors. This study was based on assessment of mosquito larvicidal activity to determine the efficacy of acetone, chloroform and methanol extracts of leaves, bark, fruits and seeds of Annona reticulata L. These extracts were tested against fourth instar larvae of Aedes aegypti, causing Dengue fever. Seven concentrations (500, 250, 125, 62.5, 31.25, 15.63 and 7 mg/l) of various extracts of plant parts were tested against the larvae. The larval mortality was recorded for 24hr. Cytotoxic activity of A. reticulata was also studied. The chromosomal abnormalities observed were of both clastogenic and non-clastogenic types. The study reveals that the leaves, bark, fruits and seeds extracts of A. reticulata possess both cytotoxic and larvicidal activities. The use of natural products that reduces mosquito populations at the larval stage can be used to vector control. In addition to addressing the needs for treatment of water near human habitation, natural products can be utilized as mosquito insecticides, which may promote localized control of persistent vector borne diseases. The study suggests the promising potential of A. reticulata L. as an alternative source of a sustainable, biodegradable and environment friendly solution for the control of dengue vector, A. aegypti.

  1. Amer, S.M. (1965): Cytolological effects of pesticides. I. Mitotic effects of N-methyl, 1-naphthyl carbamate ?Sevin?. Cytologia.,30: 175-181.
  2. Das, N. G., Goswami, D. and Rabha B. (2007): Preliminary Evaluation of Mosquito Larvicidal Efficacy of Plant Extracts.? Vect. Borne?Dis., 44: 145.
  3. Das, N. T., Raj, S. A. and Rao, R. B. V. (1968): Cytological studies in Vicia faba treated with asafoetida. Cytologia.,33: 110-111.
  4. Dutta, P., Khan, S. A., Sharma, C. K., Doloi, P., Haarika, N. C. and Mahanta, J. (1998): Distrbution of potential dengue vectors in major township along the national highways and trunk roads of northeast. Asian. J. Trop. Med. Public Health.,?29(1): 173-176.
  5. Franklin, A. E. and Cande, W. Z. (1999): Nuclear organization and chromosome segregation. Plant Cell., 11: 523-534.
  6. Gomurgen, A. N. (2000): Cytological effect of the herbicide 2, 4-D Isooctyl ester 48% on root mitosis of Allium cepa. Cytologia., 165: 383-388.
  7. Gubler, D. J. (1998): Dengue and Dengue Hemorrhagic Fever. Clin. Microbiol. Rev., 11: 480-496.
  8. Hubalek, Z. and Halouzka, J. (1999): West Nile Fever- A reemerging mosquito-borne viral diseases in Europe.? infect. Dis., 2: 519-529.
  9. ICMR Bulletin. (2003): Prospects of using herbal products in the control of mosquito vectors., 33: 1-10.
  10. Kamaraj, C., Bagavan, A., Elango, G.,?Zahir, A. A., Rajakumar, G. and Marimuthu, S. (2011):?Larvicidal activity of medicinal plant extracts against?Anopheles subpictus and?Culex tritaeniorhynchus.? J. Med.?Res.,?134: 101-106.
  11. Kaushik, R. and Saini, P. (2009): Screening of some arid region plants for larvicidal activity against?Aedes aegypti J. Vector Borne Dis., 46: 244-246.
  12. Leme, D. M. and Marin-Morales, M. A. (2009): Allium cepa test in environmental monitoring: A review on its application. Mutat. Res., 682: 71-81.
  13. Magadula, J. J., Innocent, E. I. and Otieno, J. N. (2009): Mosquito larvicidal and cytotoxic activities of 3?Annonaspecies and isolation of active principles. Medicinal Plant Res., 3(9): 674-680.
  14. Mathur, J. and Chua, N. H. (2000): Microtubule stabilization leads to growth reorientation in Arabidopsis Plant Cell., 12: 465-478.
  15. Mc Gill, M., Pathak, S. and Hsu, T. C. (1974): Effect of ethidium bromide on mitosis and chromosomes. Chromosoma.,47: 157-167.
  16. Mercykutty, V. C. and?Stephen, J. (1980): Adriamycin induced genectic toxicity as demonstrated by?Allium ceparoot tip cells. ,?45: 769-777.
  17. Mohandas, T. and Grant, W. F. (1972): Cytogenetic effect of 2, 4-D and Amitol in relation to nuclear volume and DNA content in some higher plants. J. Genet. Cytol., 14: 773-778.
  18. Morgan, D. O. (2006): The Cell Cycle: Principles of Control. New Science Press Ltd., London.
  19. Pritchard, A. J. and Van, S. J. (1997): Antibacterial activity of South African plants used for medicinal purposes. ,5: 81-87.
  20. Promsiri, S., Naksathit, A., Kruatrachue, M. M. and Thavara, U. (2006): Evaluations of larvicidal activity of medicinal plant extracts to?Aedes aegypti?(Diptera: Culicidae) and other effects on a non target fish.?Sc.,13: 179-188.
  21. Rodrigues, A. M. S., de Paula, J. E., Degallier, N., Molez, J. F. and Espindola, L. S. (2006): Larvicidal activity of some Cerrado plants extracts against?Aedes aegypti.? Am. Mosq. Cont. Assoc., 22: 314-317.
  22. Saxena, R. C., Harshan, V., Saxena, A., Sukumararan, P., Sharma, M. C. and Lakshamana, K. M. (1993): Larvicidal and chemosterilant activity of?Annona squamosaalkaloid against?Anopheles stephensi. Am. Mosq.?Control Assoc., 9:84-90.
  23. Sharma, A. K. and Sharma, A. (1960): Spontaneous and chemically induced chromosome breaks. Rev. Cytol.,10: 101-123.
  24. Sharma, M. (1980): Effect of the presence of methyl, methoxy and hydroxyl group in chemical compounds on chromosomes. J. Expt. Biol.,?18: 787-790.
  25. Soni, N. K., Oswal, P.? and?Yadav, S. K. (1982): Effect of solar eclipse on cell division in meristematic cells of? sativum?L. Cytologia.,?47: 353-358.

[Soumya S. R., Asha Ramachandran, Dinesh Raj R. and Sivaprasad A. (2017); MOSQUITO LARVICIDAL AND CYTOTOXIC STUDIES ON ANNONA RETICULATA L. Int. J. of Adv. Res. 5 (Oct). 414-419] (ISSN 2320-5407). www.journalijar.com

Asha Ramachandran
Bishop Moore College, Mavelikara

DOI:

Article DOI: 10.21474/IJAR01/5545      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5545

Download Full Paper

Download PDF No. of Downloads: 39 | No. of Views: 138