ISOLATION, SCREENING AND CHARACTERIZATION OF AMYLASE AND CELLULASE PRODUCING BACTERIAL ISOLATES FROM DIFFERENT SOIL SAMPLES.
- Department of microbiology, vaagdevi degree and pg college, warangal, telangana, india.
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Amylases and cellulases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile and paper. In the present investigation, bacteria were isolated from municipal dump sites of different regions of Telangana, screened for the production of amylase and cellulase their optimum growth conditions were determined. A total of 15 bacterial colonies were isolated from collected soil samples.Three bacterial isolates, displayed zones of clearance in starch hydrolysis test. And one bacterial isolate displayed zone of clearance in cellulase hydrolysis test. The isolates displaying maximum amylase and cellulase activity on quantitations was selected. Characteristic feature of the strains indicates that it belongs to the genus Bacillus and will be later used for further characterization. Maximum yield of amylase and cellulase was obtained after 48hours of incubation. The first three optimum pH for enzymes activity was found to be at pH 7.0 and the next optimum pH for enzyme activity was found to be at pH 5.0 and also the four optimum temperatures for the activity was found to be at 40 C
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[Manthena Navabharath, Soumya, Prajwala, Venkatesh and Manoj. (2017); ISOLATION, SCREENING AND CHARACTERIZATION OF AMYLASE AND CELLULASE PRODUCING BACTERIAL ISOLATES FROM DIFFERENT SOIL SAMPLES. Int. J. of Adv. Res. 5 (Oct). 1385-1396] (ISSN 2320-5407). www.journalijar.com
Kakatiya university
