26Feb 2018

EVALUATION OF THE LOCAL ANAESTHETIC EFFECT OF CRASSOCEPHALUM CREPIDIOIDES IN SUITABLE EXPERIMENTAL ANIMAL MODELS.

  • Post Graduate Trainee, Department of Pharmacology, RIMS, Imphal, Manipur.
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Objective: Crassocephalum crepidioides (Family: Asteraceae) is an errect, annual and succulent herb growing in many tropical regions. This plant has been used to treat indigestion, diarrhoea, headache and epilepsy by the local people. The leaves are heated and the succus is used for treating sores and fresh wounds since many years. Hence considering its use in treating wounds for many years by the local people in different parts of the world, an attempt has been made to evaluate the role of Crassocephalum crepidioides in local anaesthesia using suitable experimental animal models. Materials and methods: Methanol extract of Crassocephalum crepidioides was tested for local anaesthetic action by using the following models: (i) intracutaneous wheal in guinea pigs (ii) plexus anaesthesia in frogs. In both the models 0.9% normal saline was used as a control and 2% xylocaine was used as the standard drug. Results: The intracutaneous wheal method in guinea pigs showed 0.33% and 0.57% anaesthesia in test drug of concentrations of 10% and 20% respectively compared to 93.33% anaesthesia using the standard drug, xylocaine of 2% concentration (P<0.01). In the lumbar plexus anaesthesia method in frogs, the onset of local anaesthetic action was 5.12?0.14 and 2.09?0.12 min in the test and xylocaine treated groups respectively when compared to the control group where it was 18.13?0.29 min (P<0.001). Conclusion: The present study showed that Crassocephalum crepidioides has significant local anaesthetic property.

  1. Tripathi KD. Essentials of Medical Pharmacology. 7th ed. New Delhi:Jaypee Brothers Medical Publishers (P) Ltd;2013. 377 p.
  2. Berde CB, Strichartz GR. Local anaesthetics. In: Miller RD, Eriksson LI, Fleisher LA, et al, editors. Miller's Anaesthesia. 7th ed. Philadelphia, Pa: Elsevier, Churchill Livingstone; 2009.
  3. Katzung BG, White PF. Local anaesthetics. In: Katzung BG, Masters SB, Trevor AJ, editors. Basic and Clinical Pharmacology. 11th ed. New York, NY: McGraw-Hill Companies Inc; 2009.
  4. Musa AA, Adekomi DA, Tijani AA, Muhammed OA. Some of the effect of Crassocephalum crepidioides on the frontal cortex, kidney, liver and testis of adult male Sprague Dawley Rats: Microanatomical s European Journal of Experimental Biology 2011;1(3):228-35.
  5. Jorim RY, Korape S, Legu W, Koch M, Barrows LR, Matainaho TK, et al. An ethnobotanical survey of medicinal plants used in the eastern highlands of Papua New Guinea. J Ethnobiol Ethnomed2012;8:47-63.
  6. Dhiman A, Nanda A, Ahmad S, Narasimhan B. In vitro antimicrobial activity of methanolic leaf extract of Psidium guajava J Pharm Bioallied Sci 2011;3(2):226-9.
  7. Kumar U, Kumar B, Bhandari A, Kumar Y. Phytochemical investigation and comparison of antimicrobial screening of clove and cardamom. Int J Pharm Sci Res 2010;1(12):138-47.
  8. Shah B, Seth AK. Textbook of pharmacognosy and phytochemistry. 2nd ed. New Delhi: Elsevier; 2014.
  9. Dhanasekaran M, Abraham GC, Mohan S. Preliminary phytochemical and histochemical investigation on Kigelia pinnata International Journal of Pharma Sciences and Research 2014;5(7):413-9.
  10. Bargah RK. Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of Moringa pterygosperma Gaertn. J Pharmacogn Phytochem 2015;4(1):7-9.
  11. OECD iLibrary. OECD guidelines for the testing of chemicals, Section 4. Test no. 423: Acute Oral toxicity- Acute toxic class method. Available at: http://www.oecd-ilibrary.org/environment/test-no-423-acute-oral-toxicity-acute-toxic-class-method_9789264071001-en. Accessed August 16, 2015.
  12. Bulbring E, Wajda I. Biological comparison of local anaesthetics. J Pharmacol Exp Ther. 1945;85:78-84.
  13. Gerald Vogel H. Effect of peripheral nerve function. In: Gerald Vogel H, Bernward A, editors. Drug Discovery and Evaluation- Pharmacological Assay. 2nd ed. Heidelberg: Springer; 2008. p. 957.
  14. Boruah RN, Leclercq PA. Characterisation of the essential oil from flower heads of Spilanthes acmella. J Essential Oil Res 5;1993:693-5.
  15. Liu SS, Michel AA, Linda JR. The management of pain, Churchill living stone inc, USA 1998:447-8.
  16. Agshikar NV, Abraham G J. Pharmacology and acute toxicity of essential oil extracted from Zanthoxylum budrunga. Indian J med Res. 60;1972:757-61.

[Ananya Das. (2018); EVALUATION OF THE LOCAL ANAESTHETIC EFFECT OF CRASSOCEPHALUM CREPIDIOIDES IN SUITABLE EXPERIMENTAL ANIMAL MODELS. Int. J. of Adv. Res. 6 (Feb). 1831-1834] (ISSN 2320-5407). www.journalijar.com

Ananya Das
Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur - 795004

DOI:

Article DOI: 10.21474/IJAR01/6624      
DOI URL: http://dx.doi.org/10.21474/IJAR01/6624

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