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In vitro culture of different explants of Holostemma ada-kodien on MS medium augmented with various concentrations of growth regulators were carried out. Caulogenesis was seen in leaves and shoot tips. Schizogenesis was observed in shoot tips and nodal segments, inoculated in MS medium fortified with NAA and BAP or KIN. Among these, shoot tips inoculated in MS medium supplemented with NAA (0.5mg L-1) and KIN (1.5mg L-1) showed highest percentage of regeneration. Rooted plantlets were transplanted.
[G.Pushparajan and Sanoop Surendran (2014); Studies on medicinal plants. 4. Micropropagation of Holostemma ada- kodien Schult.- a rare medicinal plant Int. J. of Adv. Res. 2 (8). 0] (ISSN 2320-5407). www.journalijar.com
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