Persistence and Enzymatic Activities Assessments of Larvicidal Crystal Proteins from Bacillus thuringiensis Bollgard II Cotton in Bioinoculant Rhizosphere Soils

The present research aimed to investigate the ecological risk of any transgenes release of Bacillus thuringiensis (Bt) cotton (Bollgard II) and non-Bt cotton (SVPR2) in the bioinoculants (Azospirllum and phosphate solublizing bacteria) treated rhizosphere soils. Rhizosphere soil enzymes like dehydrogenase, phosphatase, urease, invertase, protease levels and insecticidal crystal proteins were assessed and were explored by using ELISA. Few significant differences in enzyme levels in Azospirillum treated rhizosphere soil compared to phosphate solublizing bacteria inoculant and ELISA was indicated that Bt cotton bollgard II expressed Cry2Ab protein on extremely very negligible amount in soil and Cry1Ac persistence was absence. That there are no significant effects of Bt cotton on both bioinoculants treated rhizosphere soil. More importantly, this study demonstrates a comprehensive system for assessing the impacts of genetically modified Bt cotton even in bioinoculant treated rhizosphere soils. This is the first report on Bt cotton in bioinoculants soils.