Detection of Norovirus in association with ATG16L1 T300A genetic variant in Crohn’s Disease
- Department of Microbiology, College of Medicine, AL-Nahrain University, Baghdad, Iraq.
- Gastroenterology and Hepatology Teaching Hospital, Baghdad, Iraq.
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In Crohn’s disease, the interaction between ATG16L1 genetic variant and Murine Norovirus infection has been proposed. This study aims to estimate the association of Human Norovirus infection with ATG16L1 genetic variant among inflammatory bowel disease patients. This Case-control study involves 35 Crohn’s disease (CD) and 40 ulcerative colitis (UC) and 35 normal subjects obtained from 3 gastroenterology centers in Baghdad. Sequence specific primer polymerase chain reaction (SSP-PCR) used for ATG16L1 genotyping. Biopsies were sectioned and Norovirus by indirect immunofluorescence staining. Among Iraqis, T300A genetic variant confirmed as a risk factor in CD than HC (OR=2.57) or UC (OR=2.76). Among IBD patients, 9 (25.7%) CD and 7 (17.5%) UC patients were infected with Norovirus. 6 (50%) of homozygous mutant (GG) have Norovirus and 2 (20%) of heterozygous (GA) while, homozygous wild type have no evidence of Norovirus in their tissue biopsies. Among G allele carriage 38.5% were positive while 9.68% among allele A carriage were Norovirus positive. Among clinical samples the interaction between host genetics and viral infection.
[Haider F. Ghazi, Nidhal A. Mohammed and Raghad J. Hussein (2015); Detection of Norovirus in association with ATG16L1 T300A genetic variant in Crohn’s Disease Int. J. of Adv. Res. 3 (Nov). 623- 628] (ISSN 2320-5407). www.journalijar.com
