Efficient plant regeneration of Green gram (Vigna radiata (L.) Wilczek) via cotyledonary explant

The seeds of mung bean variety Vamban2 were treated with different dose/conc. of physical (Gamma rays) and chemical mutagens (Ethyl methane sulphonate) to induce mutagenesis. The phenotypic responses were studied in M¬1 and M 2¬ generations and spectrum of chlorophyll mutation were worked out. There were four types of chlorophyll mutation was observed, i.e. albina, xantha, chlorina and viridis. While analyzing the result, it was observed that the mutation frequency increased with increase in the dose/concentration of mutagen. In general, the chlorophyll mutant was higher in EMS than the gamma rays treated plants. Among the various dose/conc. of mutagens, EMS showed more frequency was observed in 50mM and gamma rays at 60kR had higher mutation frequency.