12Jan 2020

IMMUNOMODULATION OF LACTOBACILLUS PLANTARAM EXPRESSING PORCINE ROTAVIRUS VP6

  • College of Animal Science and Technology Jilin University, Changchun, 13018.
  • Ministry of Agriculture, Livestock and Fisheries, P.O. Box 9152, Veterinary Complex, 131 Nelson Mandela Rd, Dar es Salaam, Tanzania.
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Rotaviruses are members of the family Reoviridae. Depending on their particular inner capsid protein sequences, porcine rotaviruses are categorized into groups A, B and C. Lactic acid bacteria (LAB) are the common term for bacteria that produce a large amount of lactic acid and ferment carbohydrates. We constructed a Lactobacillus plantarum strain with surface displayed VP6, named NC8-pSIP409-pgsA-VP6-Dcpep The adhesion and invasion ratios of NC8-pSIP409-pgsA-VP6-Dcpep on BMDCs were 36.17% and 2.23%, respectively,which were 1.56 fold and 2.42 fold increased compared with empty vector group. Furthermore, NC8-pSIP409-pgsA-VP6-Dcpep strain harboring a eukaryotic plasmid pValac-GFP was co-cultured with BMDCs for 36h and GFP expression in BMDCs was detected by FCM to determine the delivery efficiency of pValac-GFP. The results showed that the strain could significantly enhance GFP expression in BMDCs (P=0.0110). The mice were immunized using the above strains harboring pValac-GFP and the oral administration resulted in efficient expression of GFP in both PPs andMLN at different times after the last administration. In addition, the NC8-pSIP409-pgsA-VP6-Dcpep strain significantly promoted the differentiation and maturation of DCs, the percentages of IL-4+and IL-17A+ of CD4+T cells in MLN, as well as production of B220+ IgA+ B cells in the PPs.

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[Seria Masole Shonyela, Wentao Yang, Guilian Yang and Chunfeng Wang (2020); IMMUNOMODULATION OF LACTOBACILLUS PLANTARAM EXPRESSING PORCINE ROTAVIRUS VP6 Int. J. of Adv. Res. 8 (Jan). 704-718] (ISSN 2320-5407). www.journalijar.com

SERIA MASOLE SHONYELA
VETERINARY DOCTOR

DOI:

Article DOI: 10.21474/IJAR01/10352      
DOI URL: http://dx.doi.org/10.21474/IJAR01/10352

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