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The study aims at isolation, identification and preservation of rhizobia isolated from wild legumes and further to test their ability to fix nitrogen with their homologous crop legumes. A field survey was carried out in Gezira State and Shendi area for the purpose of collecting samples of wild legumes for isolation and identification of their associated rhizobia. Rhizobium strains were isolated using YEMA media and presumptive test were carried out .Seedlings of some leguminous plants representing the main cross-inoculation groups were inoculated with isolates from the wild legumes. The result showed that all collected wild legumes were found to bear nodules on their roots. Some isolates were found to be fast-growing and acid producing rhizobia while others were found to be slow-grwoing and alkaline. The cross inoculation classification of the isolates showed that isolates from Phaseolus trilobus plants can be grouped with Rhizobium leguminosarum bv.Viceae and Rhizobium spp. Isolates from Sesbania sesban and Indigofera articulata can be grouped with Rhizobium leguminosarum bv. Viceae, Rhizobium leguminosarum bv. Phaseoli and Rhizobium spp. None of the isolates can be grouped with Rhizobium leguminosarum bv. Trifoleae, Bradyrhizobium lupinus or Bradyrhizobium japonicum. It was also noticed that all isolates formed nodules on roots of Vigna unguiculata and they are belonging to the miscellaneous cowpea group. All of the RAPD primers gave amplification products and they were all reproducible. The most relative isolates were SH1 and ENRRI23 with 73% similarity; while the most distant were ENRRI21 and TAL380 with similarity percentage of 26%.
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[Tagelsir Hassan Mohamed Ahmed and Rabab Mohamed Idriss Musa. (2017); DIVERSITY, COMPTABILITY AND NITROGEN FIXATION OF WILD LEGMES RHIZOBIA. Int. J. of Adv. Res. 5 (1). 594-602] (ISSN 2320-5407). www.journalijar.com
Article DOI: 10.21474/IJAR01/2787 DOI URL: http://dx.doi.org/10.21474/IJAR01/2787
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