20Jan 2017


  • Department of Microbiology, Govt.Institute of Science, Aurangabad. (MS).India
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Tannin Acyl Hydrolase (E.C commonly referred as Tannase is one of the important hydrolytic microbial enzymes. It hydrolyses hydrolysable tannin and produces glucose and gallic acid. It is an industrially important enzyme and has several applications in various industries such as food, pharmaceutical, leather, animal feed and cosmetics. Realizing the importance of enzyme tannase, the present study aims to investigate the total phenolic content and hydrolysable tannin content from different agro-residues and utilize the efficient agro-residue as a substrate for potent tannase producing microorganism for the production of tannase. The amount of total phenols, were analyzed using a spectrophotometric technique, using Folin-ciocalteau reagent. Gallic acid was used as standard compound and the total phenols were expressed as mg/g gallic acid equivalents. The hydrolysable tannin content was analyzed by Mondal’s method. The maximum phenolic content and hydrolysable tannin content was found in Punica granatum peel (pomegranate peel) and Prunus dulcis leaves (almond leaves) respectively. Thus out of the thirty one agro-residues; three agro-residues were selected for final screening having higher concentration of hydrolysable tannin content viz. Prunus dulcis leaves (almond leaves), Annona reticulate leaves (Custard apple leaves); Azadirachta indica leaves (Neem leaves). The selected agro residues were used for production of microbial tannase. Accordingly the actinomycetal isolate, Streptomyces sp.SKA1 and the leaves of Azadirachta indica were selected as potent combination for tannase production.

  1. Aguilar, C. N. and Gutierrez-Sanchez, G. 2001. Review: sources, properties, applications and potential uses of tannin acyl hydrolase.Food Sci. Tech. Int. (7): 373–382.
  2. Bajpai, B., and Patil, S. A. 2008. New approach to microbial production of gallic acid. J. Microbiol.708–711.
  3. Bele, A. A., Jadhav, V. M. and Kadam, V. J. 2010. Potential of tannins: a review. Asian Journal of Plant Sciences. 9(4): 209–214.
  4. Belmares, R., Contreras-Esquivel, J. C., Rodríguez-Herrera, R., Coronel, A. R., and Aguilar, C. N., 2004. Microbial production of tannase: An enzyme with potential use in food industry. LWT—Food Science and Technology. 37(8):857–864.
  5. Banerjee, D., Mondal, K. and Bikas, R. 2001. Production and characterization of extracellular and intracellular tannase from newly isolated Aspergillus aculeatusJ.Basic Microbial. (6): 313-318.
  6. Belur, P. D., Gopal, M., Nirmala, K. R and Basavaraj, N. 2010. Production of novel cell-associated tannase from newly isolated Serratia ficaria Journal of Microbiology and Biotechnology. 20(4):732–736.
  7. Bhat, T. K., Singh, B. and Sharma, O. P. 1998. Microbial degradation of tannins-A current perspective. Biodegradation, 25: 343 357.
  8. Biswas, K., Chatopadhyay, I., Banerjee, R. K. and Bandopadhyay, U. 2002. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci.82:1336.
  9. Bors, W. and Michel, C. 1999. Antioxidant capacity of flavanols and gallate esters: pulse radiolysis studies. Free Radical Biology and Medicine 27(11-12): 1413–1426.
  10. Chae, S. K. and Yu, T. J. 1983. Experimental manufacture of acron wine by fungal tannase.Hanguk Sipkum Kwahakhoechi. (15): 326–332.
  11. Coggon, P. and Sanderson, G. W. Manufacture of instant tea. Patent Ger. offen 2.304073 (cl. A. 23f.) 16 August 1973.
  12. Das Mohapatra, P.K., Mondal, K. C.,Pati, B .R., Halder, S. K., Maity, C. and Jana, A. 2012. Rapid screening of tannase producing microbes by using natural tannin. Braz. J. Microbiol 43(3):1080-1083
  13. Das Mohapatra, P. K., Mondal, K. C. and Pati, B. R. 2006. Production of tannase through submerged fermentation of tannin-containing plant extracts byBacillus licheniformis  Pol. J. Microbiol. (55):297–301.
  14. Jean, D., Pourrat, H. Pourrat, A. and Carnat, A. 1981. Assay of tannase (tannin acyl hydrolase E.C. by gas chromatography. Biochem. (110): 369–372.
  15. Iibuchi, S., Minoda, Y. and Yamada, K. 1967.Studies on tannin acyl hydrolase a new method determining the enzyme activity using the change of ultraviolet absorption.Biol. Chem. (31): 513–518.
  16. Mondal, K. C., Banerjee, D., Jana, M. and Pati, B. R. 2001. Colorimetric assay method for determination of tannin acyl hydrolase (E.C. activity. Anal Biochem 295:168–171
  17. Lekha, P. K., and Lonsane, B. K. Production and application of tannin acyl hydrolase: state of the art. In: Neidleman S Laskin A editors. Advances in Applied Microbiology 44. San Diego, Calif, USA: Academic Press; 1997. pp. 215–260.
  18. Paranthaman, R., Vidyalakshmi, R., Murugesh, S. and Singravadivel, K. 2008. Optimisation of fermentation conditions for production of tannase enzyme by Aspergillus oryzae using sugarcane bggasse and rice straw. Global journal of Biotechnology and Biochemistry.3 (2):105-110.
  19. Pepi, M., Lampariello, L. R. and Altieri R. et al.2010. Tannic acid degradation by bacterial strains Serratia and Pantoea sp. isolated from olive mill waste mixtures. International Biodeterioration and Biodegradation. 64(1):73–80.
  20. Premakumari, K.B., Ayesha, Siddiqua, Shanaz, Banu. Josephine, J., Leno, Jenita. and Bincy, Raj. 2010. Comparative Antimicrobial Studies of Methanolic Extract of Muntingia calabura, Basella alba and Basella rubra Leaves. Research Journal of Pharmacognosy and Phytochemistry. 2(3): 246-248.
  21. Sharma, G. and Dubey, S.,al 2011. Phytochemical screening and estimation of total phenolic content in Aegle marmelos seeds. International journal of pharmaceutical and clinical research.3 (2): 27-29.
  22. Sharma, P. C., Bhatiya, V., Bansal, N.and Sharma A.2007 A review on bael tree. Natural products radiance.6 (2):171-78.
  23. Singleton, V. L., Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents.Am.J.Enol.Vitic. (16): 144-158.

[S. N. Girdhari and S. A. Peshwe. (2017); SCREENING OF AGRO-RESIDUES FOR THE PRODUCTION OF MICROBIAL TANNASE. Int. J. of Adv. Res. 5 (1). 625-631] (ISSN 2320-5407). www.journalijar.com

Sarika Girdhari
Government Institute of Science, Aurangabad, (MS), India


Article DOI: 10.21474/IJAR01/2790       DOI URL: http://dx.doi.org/10.21474/IJAR01/2790

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