Effect of ATP on Asthinozoospermic Men In Vitro

The lack of proper mobility of sperm is the main cause of male infertility. One of the ways to deal with this problem is in vitro activation of the sperm by adding some materials.Objective of the present study is to investigate the effect of different concentrations of adenosine triphosphate (ATP) on the outcome of in vitro human sperm activation of Asthinozoospermic patients. Thirty samples of semen of infertile men was shared in this experimental laboratory study, during their attendance of the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies / Al- Nahrain University with mean age was (33.61 ± 5.23) year. Sperm concentration, morphology (%) and motility (progressive, non-progressive and immotile) (%) were analyzed according to WHO guidelines. Before using centrifugation swim-up technique, the washed samples were divided into 3 groups: control group (G1) without ATP. While, in G2, G3 doses of 1.25 mM, and 2.5 mM ATP was added respectively. Moreover, all groups enriched with SMART media. The sperm parameters were evaluated after the addition of ATP the results showed a significant increase (P<0.05) in the progressive sperm motility percentage as compared to the G1. While, in G3 with dose of the 2.5 mM ATP, progressive sperm motility percentage was significantly increased (P<0.05) as compared to G1and non-significant (P>0.05) when compared with G2. Based on the results of this study, it can be concluded that the addition of 2.5 mM ATP to washed sperms can improve sperm motility of Asthinozoospermic samples. Data were analyzed statistically using complete randomized design (CRD) (one way).