20Mar 2017

COMPARISON OF MITOTIC EFFECT OF AQUEOUS, AICOHOL AND PETROLEUM ETHER EXTRACT OF THE LEAVES OF BOERHAAVIA DIFFUSA L.ON ROOT MERISTEM OF ALLIUM CEPA L.

  • Post Graduate Department Botany, Deva Matha College, Kuravilangad, Kottayam, Kerala, India
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In the present study the effects of various extracts of Boerhaavia diffusa L. on mitotic cells of Allium cepa L. is investigated. The concentrations are set as 0.05, 0.1, 0.15 and 0.2 using water, petroleum ether and ethyl alcohol extract of stem and leaves. The duration of the treatment is 30 minutes prior to mitotic peak. The mitotic index and index of various stages of mitosis are scored along with the number of abnormal cells. The mitotic index is found to be reduced after higher concentration of treatments while at lower concentrations there is an increase in the number of dividing cells. The percentage of abnormal cells is found to be increased with increase in the concentration of the treatment. The abnormalities showed a predominance of stickiness than fragments, bridges, unoriented and tropokinesis. The results indicated that the active toxic element is an ethyl alcohol soluble fraction as evidenced by higher frequency of abnormal cells. Thus it is recommended that excessive use of ayurvedic preparations like purnavasasa, a preparation made entirely from Boerhaavia diffusa L. may harm the epithelial lining of stomach and intestine.

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[Rijuraj M.P. and C. T. Chandralekha. (2017); COMPARISON OF MITOTIC EFFECT OF AQUEOUS, AICOHOL AND PETROLEUM ETHER EXTRACT OF THE LEAVES OF BOERHAAVIA DIFFUSA L.ON ROOT MERISTEM OF ALLIUM CEPA L. Int. J. of Adv. Res. 5 (Mar). 379-388] (ISSN 2320-5407). www.journalijar.com

Rijuraj M P
Post Graduate Department Botany, Deva Matha College, Kuravilangad, Kottayam

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Article DOI: 10.21474/IJAR01/3523      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3523

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