29Mar 2017

ASSESSMENT OF ENDOTHELIAL HEMOSTASIS; SERUM NITRIC OXIDE AND ENDOTHELIN-1 LEVELS IN ISCHEMIC CEREBROVASCULAR STROKE WITH OR WITHOUT TYPE 2 DIABETES.

  • Internal Medicine Department, Faculty of Medicine, Zagazig University- Egypt.
  • Medical Biochemistry Department, Faculty of Medicine, Zagazig University- Egypt. 3Neurology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
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Background: stroke is the most common cause of disability as well as the second leading cause of mortality worldwide. The role of endothelial dysfunction in stroke is critical. Nitric oxide (NO) and endothelin-1 (ET-1) which are produced in endothelial cells are leading molecules regulating vascular function. Aim of the work: the aim of our study was to estimate serum nitric oxide and endothelin-1 levels as biomarkers of endothelial function in ischemic cerebrovascular stroke with or without type 2 diabetes mellitus (T2DM). Methods: A cross-section study included 100 patients with ischemic stroke who were stratified into two groups according to their fasting blood glucose into non-diabetic group (n=45) and diabetic group (n=55). The levels of serum NO were determined calorimetrically. Serum ET-1 concentrations were also estimated by enzyme immunoassay technique. Results: Our results showed that non diabetic patients with ischemic stroke had significantly higher values of serum NO compared to diabetic group .On the contrary; there was highly significant elevated serum ET-1 levels in diabetic group compared to non-diabetic group. After adjusted for the traditional risk factors, logistic regression analysis test demonstrated that both serum NO and ET-1 levels were statistically significant predictors of insulin resistance among patients with ischemic stroke. Linear regression analysis test showed that serum NO levels were independently correlated with high density cholesterol (HDL.C) values and systolic blood pressure. Regarding serum ET-1 levels, they were independently correlated with homeostasis model assessment of insulin resistance index (HOMA-IR) and waist/hip ratio (WHR) in patients with ischemic stroke. Conclusions: Both serum endothelin-1 and nitric oxide levels could be useful diagnostic biomarkers predicting insulin resistance among patients with ischemic stroke.


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[Nearmeen M. Rashad, Neveen G. Elantouny, Aml S. El-Shal, Hala A. Fathy, Tarek M. H. Ibrahim, Nahawand A. El Deeb and Azza H. Abd El-Fatah. (2017); ASSESSMENT OF ENDOTHELIAL HEMOSTASIS; SERUM NITRIC OXIDE AND ENDOTHELIN-1 LEVELS IN ISCHEMIC CEREBROVASCULAR STROKE WITH OR WITHOUT TYPE 2 DIABETES. Int. J. of Adv. Res. 5 (3). 1391-1399] (ISSN 2320-5407). www.journalijar.com


Nearmeen M. Rashad
Associate Professor Internal medicine,Diabetes and Endocrinology

DOI:


Article DOI: 10.21474/IJAR01/3646       DOI URL: http://dx.doi.org/10.21474/IJAR01/3646


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