22Apr 2017

OPTIMIZATION STUDIES ON ALKALINE PROTEASE FROM ASPERGILLUS TERREUS ISOLATED FROM MARINE SOIL.

  • Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, A.P.
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Extracellular proteases have high commercial value and multiple application in detergents, food, dairy, pharmaceutical, leather, diagnostics, waste management and several recovery industries. The Aspergillus terreus isolated from kottakoduru seashore, Nellore, was found to produce alkaline protease. Maximum enzyme production was recorded at pH 8 and temperature of 30 oC in a medium containing 10% wheat bran. ZnSO4 is effective activator for protease activity and PMSF had given approximately 50% inhibition, indicating that it is a serine protease. Sesame oil cake proved to be suitable substrate after wheat bran for the production of protease by Aspergillus terreus.

  1. Anwar, A. and Saleemuddin, M., 2000. Alkaline protease from Spilosoma obliqua: potential applications in bio‐Biotechnology and applied biochemistry,?31(2), pp.85-89.
  2. Banerjee, U.C., Sani, R.K., Azmi, W. and Soni, R., 1999. Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive.Process biochemistry,?35(1), pp.213-219.
  3. Borriss R. 1987. Biology of enzymes. In: (Rehm H & Reed G. eds) Biotechnology. Weinheim, Verlag chemie. pp. 35-62.
  4. Chakrabarti, S.K., Matsumura, N and Ranu R.S, 2000. Purification and characterization of an extracellular alkaline serine protease from A. terreus, Curr Microbiol, 40: 239-244.
  5. Chandrasekaran, S., Kumaresan, S.S.P. and Manavalan, M., 2015. Production and Optimization of Protease by Filamentous Fungus Isolated from Paddy Soil in Thiruvarur District Tamilnadu.Journal of Applied Biology and Biotechnology Vol,?3(06), pp.066-069.
  6. Chellapandi, P., 2010. Production and preliminary characterization of alkaline protease from Aspergillus flavus and Aspergillus terreus.Journal of Chemistry,?7(2), pp.479-482.
  7. Dubey, R., Adhikary, S., Kumar, J. and Sinha, N., 2010. Isolation, production, purification, assay and characterization of alkaline protease enzyme from Aspergillus niger and its compatibility with commercial detergents.Dev Microbiol Mol Biol,?1(1), pp.75-94.
  8. Ellaiah, P., Srinivasulu, B. and Adinarayana, K., 2002. A review on microbial alkaline proteases.
  9. Ghildyal, N.P., Lonsane, B.K., Sreekantiah, K.R. and Sreenivasa Murthy, V., 1985. Economics of submerged and solid state fermentations for the production of amyloglucosidase.Journal of Food Science and Technology,?22(3), pp.171-176.
  10. Gopalkumar G. Rao, Priyanka M. Patel, B.V. Rao?and Rakeshkumar R. Pancha. 2016. Alkaline protease production by?Aspergillus terreusBAB-346 using poultry litter waste.? J. Curr. Microbiol. App. Sci.,? 5(10):174-184.
  11. Gupta, R., Beg, Q. and Lorenz, P., 2002. Bacterial alkaline proteases: molecular approaches and industrial applications.Applied microbiology and biotechnology,?59(1), pp.15-32.
  12. Hossain, M.T., Das, F., Marzan, L.W., Rahman, M.S. and Anwar, M.N., 2006. Some properties of protease of the fungal strain Aspergillus flavus.International Journal of Agriculture and Biology (Pakistan).
  13. Hussain, Ahan, Mannam, Abdul,Zubair,Hursan et al.,(2010). Purification and characterization of Alkaline proteases from Aspergillus terreus.Journal of chemical society of Pakistan.32(4)497-504.
  14. Ishikawa, H., Ishimi, K., Sugirura, M., Sowa, A.and Fujiwara, N.(1993).Kinetics and Mechanisms of enzymatic hydrolysis of gelatin of x-ray film and release of silver particles.Ferment.Bioeng.76:300-30
  15. Joo, H.S., Kumar, C.G., Park, G.C., Kim, K.T., Paik, S.R. and Chang, C.S., 2002. Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii.Process Biochemistry,?38(2), pp.155-159.
  16. Kaur, S., Vohra, R.M., Kapoor, M., Beg, Q.K. and Hoondal, G.S., 2001. Enhanced production and characterization of a highly thermostable alkaline protease from Bacillus sp. P-2.World Journal of Microbiology and Biotechnology,?17(2), pp.125-129.
  17. Kirk, O., Borchert, T.V. and Fuglsang, C.C. (2002). Industrial enzymes applications. Curr. Opin. Biotechnol. 43: 473-481.
  18. Kranthi, V.S., Rao, D.M. and Jaganmohan, P., 2012. Production of protease by Aspergillus flavus through solid state fermentation using different oil seed cakes.International Journal of Microbiological Research,?3(1), pp.12-15.
  19. Oskouie, S.F.G., Tabandeh, F., Yakhchali, B. and Eftekhar, F., 2008. Response surface optimization of medium composition for alkaline protease production by Bacillus clausii.Biochemical Engineering Journal,?39(1), pp.37-42.
  20. Osman, M.E., Khattab, O.H. and Elsaba, Y.M. Aspergillus terreus Proteases: Characterization and Applications. J. Chem. Bio. Phy. Sci. Sec. B,4 (3) : 2333-2346
  21. Mukhtar, H., Ali, Z. and Riaz, N., 2004. Protease biosynthesis by mutant strain of Penicillium griseoroseum and cheese formation.Pakistan Journal of Biological Sciences (Pakistan).
  22. Muthulakshmi, C., Gomathi, D., Kumar, D.G., Ravikumar, G., Kalaiselvi, M. and Uma, C., 2011. Production, purification and characterization of protease by Aspergillus flavus under solid state fermentation.Jordan J. Biol. Sci,?4(3), pp.137-148.
  23. Naidu, K.S.B. and Devi, K.L., 2005. Optimization of thermostable alkaline protease production from species of Bacillus using rice bran.African Journal of Biotechnology,?4(7), pp.724-726.
  24. Niyonzima, F.N. and More, S.S., 2015. Purification and characterization of detergent-compatible protease from Aspergillus terreus gr.3 Biotech,?5(1), pp.61-70.
  25. Pagare, R.S., Ramdasi, A.M., Khandelwal, S.R., Lokhande, M.O. and Aglave, B.A., 2009. Production and enzyme activity of an extracellular protease from Aspergillus niger and Bacillus subtilis.International Journal of Biotechnology & Biochemistry,?5(3), pp.3-8.
  26. Paranthaman, R., Alagusundaram, K. and Indhumathi, J., 2009. Production of protease from rice mill wastes by Aspergillus niger in solid state fermentation.World Journal of Agricultural Sciences,?5(3), pp.308-312.
  27. Racheal, O.O., Ahmed, A.T.F., Ndigwe, E.V. and Morakinyo, S.D., 2015. Extraction, purification and characterization of protease from Aspergillus Niger isolated from yam peels.International Journal of Nutrition and Food Sciences,?4, pp.125-131.
  28. Ramchandra, S., Singh, S., Larroche, C., Soccol, C., Pandey, A. 2007. Oil cakes and their Biotechnological applications - A review, Biores. Technol., 98: 2000-2009
  29. Sabu, A., Sarita, S., Pandey, A., Bogar, B., Szakacs, G. and Soccol, C.R., 2002. Solid-state fermentation for production of phytase by Rhizopus oligosporus.Applied Biochemistry and Biotechnology,?102(1), pp.251-260.
  30. Sharma, J., Singh, A., Kumar, R. and Mittal, A., 2006. Partial purification of an alkaline protease from a new strain of Aspergillus oryzae AWT 20 and its enhanced stabilization in entrapped Ca-Alginate beads.Internet J Microbiol,?2(2), pp.1-14.
  31. Srilakshmi, J. Madhavi, J and Ammani. K. 2014. .Protease production by Humicola grisea through SSF.Global Journal of Bio-Science and Biotechnology. 3(3): 230-235.
  32. Sumantha, A., Sandhya, C., Szakacs, G., Soccol, C.R. and Pandey, A., 2005. Production and partial purification of a neutral metalloprotease by fungal mixed substrate fermentation.Food Technology and Biotechnology,?43(4), pp.313-319.
  33. Tremacoldi C.R., R. Monti. H. S. Selistre-De-Arau?jo . E. C. Carmona. 2006. Purification and properties of an alkaline protease of Aspergillus clavatus. World J Microbiol Biotechnol. 23:295?299.
  34. Takami, H., Akiba, T. and Horikoshi, K., 1990. Characterization of an alkaline protease from Bacillus sp. no. AH-101.Applied microbiology and biotechnology,?33(5), pp.519-523.

[Suman Kumar and M. Raghu Ram. (2017); OPTIMIZATION STUDIES ON ALKALINE PROTEASE FROM ASPERGILLUS TERREUS ISOLATED FROM MARINE SOIL. Int. J. of Adv. Res. 5 (Apr). 300-306] (ISSN 2320-5407). www.journalijar.com

mraghuram
Acharya Nagarjuna Univeristy

DOI:

Article DOI: 10.21474/IJAR01/3811      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3811

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