25Apr 2017

FASUDIL HYDROCHLORIDE EFFECTS ON EAAT2 AND ROCK2 PROTEIN EXPRESSIONS IN RAT MODEL OF NEONATAL HYPOXIC-ISCHEMIC ENCEPHALOPATHY.

  • Department of pediatrics unit 1,First Affiliated Hospital of Jiamusi University.
  • Professor and Head, Department of pediatrics unit 1, First Affiliated Hospital of Jiamusi University.
  • Department of Gynecology and Obstetrics, First Affiliated Hospital of Jiamusi University.
  • School of Stomatology, Department of Orthodontics and Dentofacial Orthopedics, Second Affiliated Dental Hospital of Jiamusi University.
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Objective: To evaluate fasudil effects on EAAT2 and ROCK2 protein expressions in rat model of neonatal hypoxic-ischemic brain damage (HIBD). Methodology: 144 Seven-day-old Wistar rats were randomly divided into sham, HIBD, and fasudil groups. Fasudil group were injected intraperitoneally with that compound. Rat brains at 2, 6,12, 24, 48 and 72 h after HIBD were collected to determine histopathological damage and the expression levels of EAAT2 and ROCK2 proteins selected as the average optical density (AOD).Results were reported as average (x)standard deviation (SD), and single-factor analysis of variance (ANOVA) was performed between the multiple groups. A value of P <0.05 was considered statistically significant. Results: Histopathological damage was reduced in fasudil groups compared with the untreated HIBD group. The expression of ROCK2 in the HIBD group was significantly higher than that in SHAM and fasudil groups at the same times. EAAT2 expression was significantly decreased in HIBD group. Fasudil increased that expression. Conclusion: Fasudil reduced expression of Rock2 and increased expression of EAAT2. The increase in the expression of EAAT2 constitutes one of the pathways by which fasudil acts and that can explain its neuroprotective effects. Nevertheless further investigation should be conducted to better understand the mechanism of action of fasudil in neuroprotection it could bring in this disease.

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[Ndefi A ntima Yadiswa Robert, Wang Xian He, Zhang Ya Li, Zhu Zhang Long, Raihan Kibria, Nalika Robai and Indranil Ghosh. (2017); FASUDIL HYDROCHLORIDE EFFECTS ON EAAT2 AND ROCK2 PROTEIN EXPRESSIONS IN RAT MODEL OF NEONATAL HYPOXIC-ISCHEMIC ENCEPHALOPATHY. Int. J. of Adv. Res. 5 (Apr). 1674-1679] (ISSN 2320-5407). www.journalijar.com

Ndefi A’ntima Yadiswa Robert
jiamusi

DOI:

Article DOI: 10.21474/IJAR01/3997      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3997

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