10Sep 2017

EVALUATION OF THE CHARACTERISTICS OF YEAST SPECIES ISOLATED FROM THE TERMITE MACROTERMES SUBHYALINUS AND THEIR POTENTIAL TO PRODUCE SORGHUM BEER.

  • Laboratoire de Biocatalyse et des Bioproc?d?s de l?Universit? Nangui Abrogoua (Abidjan, C?te d?Ivoire), 02 BP 801 Abidjan 02, C?te d?Ivoire.
  • Centre National de Recherche Agronomiques, KM17 Route de Dabou, 01 BP 1740 Abidjan 01 (C?te d\'Ivoire).
  • Laboratoire de Biotechnologie et de Microbiologie des Aliments de l?Universit? Nangui Abrogoua (Abidjan, C?te d?Ivoire), 02 BP 801 Abidjan 02, C?te d?Ivoire.
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In the way to found the best starter cultures for sorghum beer production, three yeast strains [one Issatchenkia orientalis (L1) and two Candida tropicalis (L2 and L6)] isolated from the digestive tract of termite Macrotermes subhyalinus were tested in pure culture and co-culture (L1-L2, L1-L6, L2-L6 and L1-L2-L6) in the production of sorghum beer. The effect of initial pH on the growth and acidifying power, the ability to grow in the presence of ethanol, the evolution of biomass and the physicochemical properties of the beers produced were determined. The results showed that all the starters had the ability to grow at acid (3; 4; 5.5), neutral (7) as basic (9) pH conditions but the highest growth varied with the culture medium initial pH. They had an acidifying capacity which was better expressed when the initial pH was greater than 5. The starter L1 was the best acidifying starter at all initial pH with values of 0.4, 1.3, 2.6 and 4.1, respectively at pH 4, 5.5, 7 and 9. Their growth was not completely inhibited by the ethanol up to 7.5% but the starter which better face to ethanol toxicity varied according to incubation time. These starters had the capacity to grow in sorghum sweet wort and fermented it at temperatures of 30?C, 35?C and 40?C. The principal component analysis revealed that the beers obtained at 30?C and 35?C with starters L2-L6, L1-L2-L6 on one hand and L1-L2, L1-L6, L2 on the other hand had similar characteristics.


  1. Adewara, A.O. and Ogunbanwo, S.T. (2013): Effects of processing variables on the production of ?Burukutu?, a Nigerian fermented beverage. Nat. Sci., 11: 16-28.
  2. Aldiguier, A.S. (2006): Activit? bio-catalytique en haute densit? cellulaire de Saccharomyces cerevisiae pour l?intensification de la production de bio?thanol. Th?se INSA-Toulouse, France, 277p.
  3. Alexandre, H., Rousseaux, I., and Charpentier, C. (1994): Ethanol adaptation mechanisms in Saccharomyces cerevisiae. Biotechnol. Appl. Biochem., 20: 173?183.
  4. Bernfeld, P. (1955): Amylase α and β. In: Colswick, S.P. and Kaplan N.O. (eds) Methods in enzymology: Academic Press Inc, New-York, pp. 149-154.
  5. Carrasco, P., Querol, A. and del Olmo, M.L. (2001): Analysis of the stress resistance of commercial wine yeast strains. Arch. Microbiol., 175: 450-457.
  6. Casey, G.P. and Ingledew, W.M. (1986): ? Ethanol tolerance in yeast N, CRC Critical Rev Microbiol, 13: 219-280.
  7. Chevassus-Agnes, S., Favier, J. C. and Joseph, A. (1976): Technologie traditionnelle et valeur nutritive des bi?res de sorgho du Cameroun. Nutr. Di?t., 11: 89-104.
  8. Coulibaly, W. H. (2016): Mise en place d?un starter lyophilis? pour la fermentation alcoolique de la bi?re de sorgho. Th?se, Universit? Nangui Abrogoua, Abidjan, C?te d?Ivoire, 170p.
  9. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956): Colorimetric method for determinations of sugars and related subtances. Ana. chem. lett., 280: 350-356.
  10. Gabriel, P., Dienstbie, R.M., Matoulkov?, D., Kosař, k. and Sigler, K. (2008): Optimised acidification power test of yeast vitality and its use in brewing Practice. Inst. Brew., 114: 270?276.
  11. Glover, R.L.K., Abaidoo, R.C., Jakobsen, M. and Jespersen, L. (2005): Biodiversity of Saccharomyces cerevisiae isolated from a survey of pito production sites in various parts of Ghana. Appl. Microbiol., 28: 755?761.
  12. Glover, R.L.K., Sawadogo- Lingani, H., Diawara, B., Jespersen, L. et Jakobsen, M. (2009): Utilization of Lactobacillus fermentum and Saccharomyces cerevisiae as starter cultures in the production of ?dolo?. J. Appl. Biosci., 22: 1312-1319.
  13. Haggblade, S. and Holzapfel W.H. (1989): Industrialization of Africa?s indigenous beer brewing. In: Steinkraus KH (eds) Industrialization of indigenous fermented foods. Marcel Dekker, New York, pp. 91?283.
  14. Holzapfel, W.H. (2002): Appropriate starter culture technologies for small-scale fermentation in developing countries. Int. J. Food. Res., 75: 197?212.
  15. Jones, R.P. and Greenfield, P.F. (1987): Specific and non-specific inhibitory effect of ethanol on yeast growth. Enzyme Microb. Technol., 9: 334-338
  16. Karima, B. (2008): Etude de l?alpha amylase de levures isol?es d?un ?cosyst?me extr?me (sol environnant des sources thermales) et cultiv?es sur un milieu ? base de lactos?rum. Th?se, Universit? Mentouri Constantine, R?publique populaire et d?mocratique d?Alg?rie, 104p.
  17. Maoura, N., Mbaiguinam, M., Nguyen, H.V., Gaillardin, C. and Pourquie, J. (2005): Identification and typing of yeast strains isolated from bili bili, traditional sorghum beer of Chad. J. Biotechnol., 4: 646-656.
  18. Maoura, N., Mbaiguinam, M., Nguyen, H.V., Gaillardin, C. and Pourquie, J. (2006): Suivi technique, analytique et microbiologique du bili bili, bi?re traditionnelle tchadienne. Sci., 2: 69-82.
  19. N?da, A.K. and Coulibaly, S. (1996): Etude comparative de proc?d?s traditionnels de pr?paration d?une bi?re locale appel?e tchapalo. 2i?me journ?es annuelles de la SOACHIM, Dakar, pp. 40-46.
  20. N?guessan, F.K., N?Dri, D.Y., Camara, F. and Dj?, M.K. (2010): Saccharomyces cerevisiae and Candida tropicalis as starter cultures for the alcoholic fermentation of tchapalo, a traditional sorghum beer. World J. Microbiol. Biotechnol., 26: 693?699.
  21. Odumeru, J.A., D?Amore, T., Russell, I. and Stewart, G.G. (1993): Alterations in fatty acid composition and trehalose concentration of Saccharomyces brewing strains in response to heat and ethanol shock. Ind. Microbiol. Biotechnol., 1: 113?119.
  22. Opekarova, M. and Sigler, K. (1982): Acidification Power: Indicator of Metabolic Activity and Autolytic Changes in Saccharomyces cerevisiae. Folia Microbiol., 27: 395-403.
  23. Quoc, Phong, LA. (2010): Utilisation de levures non Saccharomyces en oenologie : ?tudes des interactions entre Torulaspora delbrueckii et Saccharomyces cerevisiae en cultures mixtes. Th?se, Universit? de Toulouse, France, 161p.
  24. Sawadogo-Lingani, H., Lei, V., Diawara, B., Nielsen, D.S., M?ller, P.L., Traor?, A.S. and Jakobsen M. (2007): The biodiversity of predominant lactic acid bacteria in dolo and pito wort, for production of sorghum beer. Appl. Microbiol., 103: 765?777.
  25. Sawadogo-Lingani, H., Diawara, B., Traor?, A.S. and Jakobsen, M. (2008): Utilisation de souches s?lectionn?es de Lactobacillus fermentum et un isolat de levure comme cultures starters dans la production du dolo, une boisson ferment?e ? base de sorgho. Tech., 2: 61-84.
  26. Sefa-Dedeh, S., Sanni, A.I., Tetteh, G. and Sakyi-Dawson, E. (1999): Yeasts in the traditional brewing of pito in Ghana. World J. Biotechnol., 15: 593-597.
  27. Strehaiano, P., Nehme, N., Renouf, V., Lonvaud-Funel, A., Mathieu, F. and Taillandier, P. (2008): Microbial interactions in winemaking. In ? bioprocesses in food industry ? APIAsiatech (eds) pp 274-289.
  28. Van der Aa K?hle, A., Jespersen, L., Glover, R.L.K., Diawara, B. and Jakobsen, M. (2001): Identification and characterization of Saccharomyces cerevisiae strains isolated from West African sorghum beer. Yeast, 18: 1069-1079.
  29. White, L.R., Richardson, A.J., Schiewe, A. J. and White, C.E. (2003): Comparison of yeast viability/vitality methods and their relationship to fermentation performance. In: Smart K. (Second eds). Brewing yeast fermentation performance: Blackwell science, oxford Uk, pp. 138 - 148.
  30. Yao, A.A., Egounlety, M., Kouam?, L. P. and Thonart, P. (2009): Les bact?ries lactiques dans les aliments ou boissons amylac?s et ferment?s de l?Afrique de l?Ouest: leur utilisation actuelle. Annales de M?decine V?t?rinaire, 153: 54-65.
  31. Zangu?, S.C., Desobgo1., Fantha, Y. Naponni1. et Emmanuel, J. (2012): Caract?risation des mo?ts et bi?res du sorgho Safrari houblonn?s avec Vernonia amygdalina et Nauclea diderrichii. J.I.A.S., 2: 83-91.

[Philom?ne Zran, Thierry D?zay Yoro, Souma?la Dabonn? and Kouadio Florent N?Guessan. (2017); EVALUATION OF THE CHARACTERISTICS OF YEAST SPECIES ISOLATED FROM THE TERMITE MACROTERMES SUBHYALINUS AND THEIR POTENTIAL TO PRODUCE SORGHUM BEER. Int. J. of Adv. Res. 5 (9). 46-59] (ISSN 2320-5407). www.journalijar.com


Kouadio Florent N\\\'GUESSAN
Laboratoire de Biotechnologie et de Microbiologie des Aliments de l’Université Nangui Abrogoua, UFR-STA, Abidjan, Côte d’Ivoire

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Article DOI: 10.21474/IJAR01/5299       DOI URL: http://dx.doi.org/10.21474/IJAR01/5299


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