10Sep 2017

CYTOTOXICITY EVALUATION OF BIOACTIVE FRACTION FROM TERMINALIA BELLIRICA(GAERTN.)ROXB. FRUITS IN L929 CELLS.

  • School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India.
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Terminalia bellirica(Gaertn.)Roxb. (Family: Combretaceae) is a well known medicinal plant used in various traditional herbal formulations used to cure several diseases. The dried fruit of Terminalia bellerica has an important place in the traditional Indian system of medicine and forms a major ingredient of several medicaments including ?Triphala-? used as general health tonic in Ayurveda. The current study aims to investigate the cytotoxic effect of bioactive ethyl acetate fraction of T.bellirica fruits (EFTB) on L929 cell line. The toxic changes imposed by varying concentrations of the fraction were evaluated by different techniques including MTT, neutral red and LDH leakage assay. The in vitro toxicity studies showed that EFTB, even at its highest concentration of 100 μg/ml used in the study exhibited only mild level of toxicity in L929 cells. The LD50 values of EFTB in all the cytotoxicity assays were found comparatively higher which points to the non- toxic nature of the plant derived bioactive fraction that can be developed into herbal therapeutic agent after further detailed in vivo toxicity studies.

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[Kuriakose Jayesh, Lal Raisa Helen, A Vysakh, Eldhose Binil and *M. S. Latha. (2017); CYTOTOXICITY EVALUATION OF BIOACTIVE FRACTION FROM TERMINALIA BELLIRICA(GAERTN.)ROXB. FRUITS IN L929 CELLS. Int. J. of Adv. Res. 5 (Sep). 120-126] (ISSN 2320-5407). www.journalijar.com

MS Latha
School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India.

DOI:

Article DOI: 10.21474/IJAR01/5309      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5309

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