Detection of hemolysin genes producing Enterohemorrhagic Escherichia coli isolated from sheep by Multiplex PCR technique

Abstract: Enterohemorrhagic Escherichia coli (EHEC) is a bacterium that can cause severe food borne disease. Primary sources of outbreaks are raw or undercooked ground meat products, raw milk and fecal contamination of vegetables. It can cause severe gastrointestinal disease, including fatal infections, and is being detected more frequently worldwide. In this study we used Multiplex Polymerase chain reaction technique (PCR) for detection hemolysin toxin genes (hlyA) and (hlyB) as virulence factors producing by Enterohemorrhagic Escherichia coli (EHEC) isolated from diarrheic sheep. The PCR primers for hlyA and hlyB gene were designed by this study from NCBI-Genbank published sequence (Genbank: AF037579.1, AM690761.1). The Multiplex PCR results was show (34) positive isolates out of 50 isolates at (68%), (20) isolates producing hlyA gene and (14) isolates producing hlyB gene whereas, (16) isolates not producing hemolysin toxin gene. In conclusion, hemolysin toxin gene (hlyA) and (hlyB) gene is important virulence factors producing by Enterohemorrhagic Escherichia coli infected sheep and used Multiplex PCR was appeared very sensitive and highly specific assay serve as suitable molecular diagnostic tool for detection Enterohemorrhagic Escherichia coli producing hemolysin toxins.

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[Dr. Fadi Jawad Hamza Al-Shammari (2014); Detection of hemolysin genes producing Enterohemorrhagic Escherichia coli isolated from sheep by Multiplex PCR technique Int. J. of Adv. Res. 2 (6). 0] (ISSN 2320-5407). www.journalijar.com

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