13May 2018

BIOREMEDIATION OF MUNICIPAL SEWAGE WATER WITH AZOLLA MICROPHYLLA

  • Research Scholar, Kerala Forest Research Institute, Peechi.
  • Senior Scientist, Kerala Forest Research Institute, Peechi.
  • Senior Scientist, Indian Agricultural Research Institute, New Delhi.
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Population rise coupled with urbanization and industrialization has resulted in over exploitation of ground water resources and lowering of the water table in many areas. The availability of clean water is at stake when the modern agricultural activities demands more water for irrigational activities. Various treatment systems which are a combination of physical, chemical or biological processes are used for purification and recycling of water. But these are expensive and unaffordable to common man. Bioremediation is an excellent, low cost option to ward off the pollutants. It has been successfully used in constructed wetlands, an ecosystem with the assemblage of plants, microbes and the abiotic factors. The paper analyses the ability of Azolla to remove nutrients from secondary treated sewage water which if released directly to water bodies result in eutrophication. The present study analyses the nutrient removal capacity of Azolla from waste water and shows that the growth of Azolla remove 54.8 per cent ammonia, 50 percent total organic carbon, 33.3 percent organic matter, 71.4 per cent nitrite, 68.65 per cent available phosphorous and 80.52 per cent total phosphorous from wastewater and prevents the eutrophication in water bodies.


  1. Arora, A., Pabby, A., Singh, P, K. (2003). Heavy metal accumulation by Azolla in contaminated soils. Proc. 18th Intl. Conf. on Contaminated Soil Sediments and Water), Vol 8 Amherst Scientific Publishers, Amherst Scientific Publishers Amherst, USA. 8, 39-48
  2. Arora, A., Saxena S. (2005). Cultivation of Azolla microphylla biomass on secondary-treated Delhi municipal effluents. Biomass and Bioenergy. 29, 60-64
  3. Arora, A., Saxena, S., Shah, R. (2009). Aquatic microphyte Azolla for Nutrient Removal from Wastewaters in Constructed Wetlands. Proceedings of International Conference on Energy and Environment. Environmental Energy. 185-188
  4. Arora, A., Singh, P, K. (2003). Comparison of biomass productivity and nitrogen fixing potential of Azolla Biomass and Bioenergy. 24, 175-178.
  5. Bassi, N., Kumar, M, D., Sharmac, A, P. Saradhi,P. (2014). Status of wetlands in India: A review of extent, ecosystem benefits, threats and management strategies. Journal of Hydrology. 2, 1-19
  6. Central Pollution Control Board. (2008). Status of water quality in India 2007, New Delhi, India: CPCB.
  7. Dawar,S., Singh, P, K. (2002). Comparison of Soil- And Nutrient-Based Medium for Maintenance of Azolla Cultures. Journal of Plant Nutrition. 25(12), 2719-2729
  8. DeBusk, T. A., Reddy, K, R. Hayes T, D., Schwegler, B, R. (1989). Performance of a pilot-scale water hyacinth-based secondary treatment system. Journal WPCF, 61, 1217?1224.
  9. Elmachliy, S., Chefetz, B., Vidal, L., Canals, A., Gedanken, A. (2010). Removal of silver and lead ions from water wastes using Azolla filiculoides, an aquatic plant, which adsorbs and reduces the ions into the corresponding metallic nanoparticles under microwave radiation in 5 min. Water Air Soil Pollution. 365-370
  10. Fisher, J. P. (1988). Wastewater treatment using aquatic plants. In (eds), Alternative Waste Treatment Systems, Rao Bhamidimarri, Elsevier Applied Science, Palmerston North, New Zealand: 34? 44.
  11. Jaeger, E, C. (1978). A Source-Book of Biological Names and Terms, 3rd Charles C Thomas, Publisher, Sringfield, III. 32
  12. Kitoh, S., Shiomi, N., Uheda, E. (1993). The growth and nitrogen fixation of Azolla filiculoides Lam. in polluted water. Aquatic botany. 46 ,129-139
  13. Kollah, B., Patra1, a, k., Mohanty, S, R. (2016). Aquatic microphylla Azolla: a perspective paradigm for sustainable agriculture, environment and global climate change. Environ Sci Pollut Res. 23:4358?4369
  14. Lumpkin, T, A., Plucknett, D, L. (1980). Azolla: Botany, physiology, and use as a green manure. 34 (2), 111-153
  15. Millennium Ecosystem Assessment (MA), (2005). Ecosystems and Human Well-being: Biodiversity Synthesis. World Resources Institute, Washington, DC.
  16. Mujiyo, Sunarminto, B, H., Hanudin, E., Widada, J., Syamsiyah, J. (2016). Methane Emission on Organic Rice Experiment Using Azolla. International Journal of Applied Environmental Sciences. 11 (1), 295-308.
  17. Noorjahan C, M., Jamuna, S. (2015). Biodegradation of Sewage Waste Water Using Azolla Microphylla and Its Reuse for Aquaculture of Fish Tilapia Mossambica. Journal of Environmental Science, Toxicology and Food Technology. Vol. 9(3). 75-80
  18. Oron, G. (1990). Economic considerations in wastewater treatment with duckweed for effluent and nitrogen renovation. Journal WPCF, 62, 692?696.
  19. Pabby, A., Prasanna, R., Singh, P, K. (2003). Azolla anabaena symbiosis from traditional agriculture to biotechnology. Indian Journal of Biotechnology. Vol.2, 26-37
  20. Pandey, A., Verma, R, K., Mohan, J., Mohan, N. (2015). Utilization of Azolla aquatic plant as phytoremediation for treatment of effluent. International Journal of Applied Research. 1(2): 28-30
  21. Peters, G. A., Meeks, J. C. (1989). The Azolla-Anabaena symbiosis: basic biology. Annual Review of Plant Physiology and Plant Molecular Biology 40, 193-2 10
  22. Prasad, S, M., Singh, A. (2011). Metabolic responses ofAzolla pinnata?to cadmium stress: photosynthesis, antioxidative system and phytoremediation. Journal of Chemistry and Ecology. 27(6), 543-555
  23. Ranalli, M., Lundholm, J, T. (2008). Biodiversity and Ecosystem function in constructed ecosystems. CAB Reviews Perspectives in Agriculture Veterinary Science Nutrition and Natural Resources. 3, 1-16.
  24. Sachdeva, S., Sharma, A. (2012). Azolla: Role in Phytoremediation of Heavy Metals. Paper from Proceeding of the National Conference ?Science in Media 2012? Organized by YMCA University of Science and Technology, Faridabad, Haryana (India)
  25. Singh, P, K. (1977). Azolla fern plant-rice fertilizer and chicken feed. Kerala Karshakan. 26, 5-6.
  26. Singh, P, K. (1989). Use of Azolla in Asian Agriculture Applied Agricultural Research. 4(3), 149-161.
  27. Singh, P, K., Panigrahi, B, C.,Satpathy, K, B. (1981). Comparative efficiency of Azolla, blue green algae and other organic manures in relation to N and P availability in a flooded rice soil Pl Soil. 62, 35-44.
  28. Sood, A., Uniyal, P, L., Prasanna, R., Ahluwalia, A, S. (2011). Phytoremediation potential of Aquatic Macrophyte, Azolla. Ambio, DOI 10.1007/s13280-011-0159-z
  29. Speelman, E, N.,?Van Kempen, M, M.,?Barke, J.,?Brinkhuis, H.,?Reichart, G,J.,?Smolders A,J,?Roelofs J,G.,?Sangiorgi, F.,?de Leeuw, J,W.,?Lotter, A,F.,?Sinninghe Damst?, J,S. (2009). The Eocene Arctic Azolla bloom: environmental conditions, productivity and carbon drawdown. Geobiology. 7(2), 155-170.
  30. Standard Methods for the Examination of Water and Wastewater. (1999). American Public Health Association (APHA), American Water Works Association, Water Environment Federation
  31. Subudhi, B, P, R., Watanabe, I. (1981). Differential Phosphorus requirements of Azolla species and strains in phosphorus limited continuous culture. Soil Sci.Plant Nut. 27(2), 237-247
  32. Vymazal, J. (2010). Constructed Wetlands for Wastewater Treatment. Water .2, 530-549
  33. Wagner, G, M. Azolla: A review of its biology and utilization. (1997). Botanical Review. 63, 1-26.
  34. Watanabe, I. (1982). Azolla-Anabaena symbiosis-it?s Physiology and use in tropical agriculture. In: Microbiology of tropical Soils and Plant Productivity. 169-185.
  35. Watanabe, I., Liu, C, C. (1992). Improving nitrogen-fixing systems and integrating them into sustainable rice farming. P1. & Soil. 141, 57-67.
  36. Yadav, R, K., Abraham, G., Singh, Y V., Singh P K. (2014). Advancements in the Utilization of Azolla-Anabaena System in Relation to Sustainable Agricultural Practices. Proceedings of Indian National Science Academy 80. 2, 301-316

[Divya Soman, Anitha V and Anju Arora. (2018); BIOREMEDIATION OF MUNICIPAL SEWAGE WATER WITH AZOLLA MICROPHYLLA Int. J. of Adv. Res. 6 (May). 101-108] (ISSN 2320-5407). www.journalijar.com


Divya Soman
Research Scholar

DOI:


Article DOI: 10.21474/IJAR01/7012      
DOI URL: http://dx.doi.org/10.21474/IJAR01/7012