04Jun 2018


  • Institut Agronomique et V?t?rinaire Hassan II.
  • Soci?t? de Productions Biologiques et Parmaceutiques V?t?rinaires (Biopharma).
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Rapid diagnosis of canine parvovirus (CPV-2) infection is of key importance in management and control of the disease. The present study aims at the development and evaluation of an easy to use rapid test for the detection of canine parvovirus particles in clinical samples collected and tested during daily veterinary practice. For this purpose, the coagglutination test (COA) using Methylene blue stained anti-CPV2 antibodies sensitised staphylococcus aureus ATCC 12598 was performed to detect CPV-2 in 91 clinical samples (86 rectal swabs and 5 organ homogenates) collected between 2011 and 2015 from suspected canine clinical cases received in Moroccan veterinary clinics. The same samples were previously tested by Hemagglutination test and real-time PCR (Amrani et al., 2016), the results were compared and employed in the evaluation of COA. The results of the COA showed 79.12% (72/91) of tested samples to be positive and 20.87% (19/91) to be negative. The intensity of reaction in positive samples was scored high (3+) in 6 samples (6.6%), medium (2+) in 28 samples (30.7%) and low (1+) in 57 samples (62.6%) which occurred within a median time of 3min, 4min and 3min42s, respectively. In comparison with PCR, the COA test demonstrated a good agreement of 80.2 % and a sensitivity of 80%. The coagglutination test is a promising test allowing daily diagnostic of canine parvovirus in field, veterinary clinics and non- equipped laboratories with molecular techniques.

  1. Amrani, N., Desario, C., Kadiri, A., Cavalli, A., Berrada, J., Zro, K., Sebbar, G., Colaianni, M.L.M.L., Parisi, A., Elia, G., Buonavoglia, C., Malik, J., Decaro, N., 2016. Molecular epidemiology of canine parvovirus in Morocco. Infect. Genet. Evol. 41, 201?206. https://doi.org/10.1016/j.meegid.2016.04.005
  2. Decaro, N., Buonavoglia, C., 2012. Canine parvovirus-A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet. Microbiol. 155, 1?12. https://doi.org/10.1016/j.vetmic.2011.09.007
  3. Decaro, N., Desario, C., Elia, G., Martella, V., Mari, V., Lavazza, A., Nardi, M., Buonavoglia, C., 2008. Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c. New Microbiol. 31, 125?130.
  4. Decaro, N., Desario, C., Parisi, A., Martella, V., Lorusso, A., Miccolupo, A., Mari, V., Colaianni, M.L., Cavalli, A., Di Trani, L., Buonavoglia, C., 2009. Genetic analysis of canine parvovirus type 2c. Virology 385, 5?10. https://doi.org/10.1016/j.virol.2008.12.016
  5. Decaro, Campolo, M., Desario, C., Elia, G., Martella, V., Lorusso, E., Buonavoglia, C., 2005a. Maternally-derived antibodies in pups and protection from canine parvovirus infection. Biologicals 33, 261?267. https://doi.org/10.1016/j.biologicals.2005.06.004
  6. Decaro, Elia, G., Martella, V., Desario, C., Campolo, M., Trani, L. Di, Tarsitano, E., Tempesta, M., Buonavoglia, C., 2005b. A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs. Vet. Microbiol. 105, 19?28. https://doi.org/10.1016/j.vetmic.2004.09.018
  7. Desario, C., Decaro, N., Campolo, M., Cavalli, A., Cirone, F., Elia, G., Martella, V., Lorusso, E., Camero, M., Buonavoglia, C., 2005. Canine parvovirus infection: Which diagnostic test for virus? J. Virol. Methods 126, 179?185. https://doi.org/10.1016/j.jviromet.2005.02.006
  8. Genovn, K., Ivanov, I., 2006. Bulgarian Academy of Sciences A rapid test for detection of canine parvovirus 45?48.
  10. Kantere, M.C., Athanasiou, L. V, Spyrou, V., Kyriakis, C.S., Kontos, V., Chatzopoulos, D.C., Tsokana, C.N., Billinis, C., 2015. Diagnostic performance of a rapid in-clinic test for the detection of Canine Parvovirus under different storage conditions and vaccination status. J Virol Methods 215?216, 52?55. https://doi.org/10.1016/j.jviromet.2015.02.012
  11. Kim, Y.K., Lim, S.-I., Choi, S., Cho, I.-S., Park, E.-H., An, D.-J., 2015. A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor. J. Virol. Methods 219, 23?7. https://doi.org/10.1016/j.jviromet.2015.03.015
  12. Montassier, H.J.J., Ara?jo J?nior, J.P., Pinto, A.. A., Araujo, J.P., Pinto, A.. A., 1994. Rapid coagglutination test for the detection and typing of foot and mouth disease virus. J. Virol. Methods 50, 29?42.
  13. Prittie, J., 2004. Canine parvoviral enteritis: a review of diagnosis, management, and prevention. J. Vet. Emerg. Crit. Care 14, 167?176. https://doi.org/10.1111/j.1534-6935.2004.04020.x
  14. Rakha, G.M.H., Abdl-Haleem, M.M., Farghali, H.A.M., Abdel-Saeed, H., 2015. Prevalence of common canine digestive problems compared with other health problems in teaching veterinary hospital, Faculty of Veterinary Medicine, Cairo University, Egypt. Vet. world 8, 403?11. https://doi.org/10.14202/vetworld.2015.403-411
  15. Singh, B.R., Yadav, R.C., Singh, S.P., Sharma, V.D., 1998. Coagglutination test: A simple and rapid immunodiagnostic test for Parvovirus infection in dogs. Indian J. Exp. Biol. 36, 622?624.
  16. Ylmaz, Z., Kennerman, E., Senturk, S., Temizel, M., Aytug, N., 2002. The evaluation of cats and dogs referred to the clinics of companion animal-Internal Medicine, Veterinary Faculty, University of Uludag (1990-2000). Vet. Fak. Dergisi, Uludag Univ. 21, 23?31.
  17. Yoshimizu, M., Kimura, T., 1985. A coagglutination test with antibody-sensitized staphylococci for rapid and simple diagnosis of bacterial and viral diseases of fish. Fish Pathol. 20, 243?261. https://doi.org/10.3147/jsfp.20.243

[Ahlam Kadiri, Nadia Amrani, Khalil Zro and Jaouad Berrada. (2018); DEVELOPMENT AND ASSESSMENT OF A COAGGLUTINATION TEST FOR DETECTING CANINE PARVOVIRUS IN CLINICAL SPECIMENS. Int. J. of Adv. Res. 6 (6). 11-17] (ISSN 2320-5407). www.journalijar.com

Nadia Amrani
Institut Agronomique et vétérinaire Hassan II


Article DOI: 10.21474/IJAR01/7181       DOI URL: http://dx.doi.org/10.21474/IJAR01/7181

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