02Nov 2018


  • Plant Biotechnology laboratory, Department of Botany, Fatima Mata National College (Autonomous), Kollam-2, Kerala.
  • Plant Tissue culture and Molecular Biology Laboratory, Department of Botany, University of Kerala, Kariavattom- 695 581, Thiruvananthapuram, Kerala.
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Indigoferatinctoria Linn.has been extensively used as a source for the extraction of indigo, which is highly priced as a natural blue dye. The plant accumulates large quantities of indican, the colourless starting compound of indigo production. Indican content was analysed in calluses derived from leaf, petiole, cotyledons, stem and root explants cultured on MS medium fortified with 1mg/l 2,4-D and 0.5mg/l BA at three different phases of growth using HPLC. The stationary phase leaf callus showed a maximum of 910 ?g/gm of indican. MS medium was found to be superior over WPM in inducing indican accumulation. Among different auxins used, IAA was found to induce maximum indican accumulation. Lower concentrations of cytokinins viz. BA (0.5 mg/l), KIN (1mg/l) and 2-ip (2mg/l) were found to be effective in the production of indican. Combinatorial effect of BA along with auxins (IAA, NAA, or IBA) was found to enhance the production of indican. 2, 4-D alone or in combination with cytokinins was found to inhibit indican production. Maximum accumulation of indican was recorded in presence of IAA (1.5mg/l) and BA (0.5mg/l) from stationary phase callus. L- tryptophan (100mg/l) was found to enhance intracellular accumulation of indican.

  1. Epstein, E., Nabores, M. W. and Stowe, B. B. 1967. Origin of indigo of woad. Nature 216: 547-549.
  2. Evans, P.K. 1990. Indigofera In: Biotechnology in Agriculture and Forestry-10 Legumes and Oilseed Crops I, ed. Y. P. S. Bajaj. Springer-Verlag, London. pp. 645-656.
  3. Hagendoorn, M. J. M. , van der Plas, L. H. W. Segers, G. J. 1994. Accumulation of anthraquinones in Morindacitrifolia cell suspensions. Plant Cell Tiss. Org. Cult. 38: 227-234.
  4. Iyer, K. N. and Kolammal, M. 1992. Pharmacognosy of Ayurvedic drugs. Pharmacognosy unit, Ayurveda college, Thiruvananthapuram, Kerala. Pp: 61-71.
  5. Harkes, PAA., De Jong, P. J., Wijnsma, R., Verpoorte, R. and Van Der Leer, T. 1986. Influence of production media on Cinchona cell cultures; Spontaneous formation of B- carbolines from L- tryptophan. Plant Sci. 47: 71.Hass and Hill, 1913
  6. Jose, S. R., Geetha, M. and Nair, G. M. 2000. Production of vasicine alkaloid from AdhatodavasicaNees. through tissue culture. Proc. Nat. Sem. Phytochemicals and nutrition. 1: 73-79.
  7. Khazhakyan, Kh. K., Egibian, KV. andDevedzhyan, AG. 1986. Effect of day lenghth on growth, development and productivity of indigo in soil less culture. Doklady- AkademiiNauk-Arminskoi-SSR. 2: 84-88.
  8. Kurz, W. G. and Constabel, F. 1979. Plant cell suspension cultures and their biosynthetic potential. Microb. Technol. 1: 389-416.
  9. Lloyd, G. and McCown, B. 1981. Commercially feasible micro propagation of mountain laurel, Kalmia latifolia by the use of shoot tip culture. Int. Plant Prop. Soc.30: 421-427.
  10. Luckner, L, 1972. Secondary metabolism in plants and animals. Chapman and Hall, London. pp.312-316.
  11. Luckner, M. 1990. Structure, biosysnthesis, and metabolism of secondary products in microorganisms, plants, and animals. In: Secondary metabolism in Microorganisms, Plant, and Animals. (ed)Luckner, M. pp. 100-444. 3rd Spinger, Berlin.
  12. Murashige, T. and Skoog, F. 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures; Physiol Plant 15: 473-497.
  13. Paul Hass and Hill, T. G. 1913. An introduction to the chemistry of plant products. Longmans Green and Co, London. pp. 188-189.
  14. Rhodes, M. J. C. 1994. Physiological role of secondary metabolites in plants: some progress, many outstanding problems. Plant Physiol. 118: 237-249.
  15. Rhodes, M. J. C., Payne, J. and Robbins, R. J. 1986. Cell suspension cultures of Cinchona ledgeriana The effect of a range of auxins and cytokinins on production of quinoline alkaloids. Planta Med. 40: 226-229.
  16. Sundberg, R. J. 1970. The chemistry of indoles. Academic Press. London. pp. 431-447.
  17. Ueda, S., Kobayashi, K., Muramatsu, T., and Inouye, H. 1981. Studies on MonoterpeneGlucosides and related natural products. PlantaMedica 41: 186-191.
  18. Xu, J. F., Ying, P. Q., Han, A. M. and Su, Z. G. 1999. Enhanced salidroside production in liquid cultivated compact callus aggregates of Rhodiolasachalinensis: manipulation of plant growth regulators and sucrose. Plant Cell Tiss. Org. Cult. 55: 53-58.
  19. Yeoman, M.M. 1987. Bypassing the plant. Annals of Botany 60, Supplement 4: 157-174.

[A. S. Rubin Jose and G. M. Nair (2018); PRODUCTION OF INDICAN FROM THE CALLUS CULTURES OF INDIGOFERA TINCTORIA LINN. Int. J. of Adv. Res. 6 (11). 16-22] (ISSN 2320-5407). www.journalijar.com

Dr. A. S. Rubin Jose
Assistant professor, Department of Botany, FMN College, Kollam, Kerala, India


Article DOI: 10.21474/IJAR01/7965       DOI URL: http://dx.doi.org/10.21474/IJAR01/7965

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