01Jan 2019


  • Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Malaysia.
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Therapeutic properties of the medicinal plant are due to the presence of phytochemical constituents. Garciniaatroviridis is locally known as ?asamgelugur? belongs to the Guttiferae family. Bioassay-guided solvent-solvent extraction method and yielded, hexane, dichloromethane, ethyl acetate, butanol, and methanolic extracts. These extracts were used to investigate the presence of phytochemicals. The preliminary phytochemical screening showed the existence of fixed oils and fats, carbohydrate, saponins, phenolic, flavonoids and anthraquinone glycosides in G. atroviridis roots. The chemical compositions were investigated by using Gas Chromatography-Mass Spectroscopy (GC-MS). Major compound identified in hexane, DCM, EA, BuOH and methanolic extracts was (Z)-13-docosenoic acid methyl ester (24.32%), ethyl-9-hexadecenoate (6.36%),bis(1,3-diisopropylcyclopentadienyl) (12.09%), 2-methyl-2-phenyl- 1,3-dioxolane (2.34%) and furfural (33.55%) respectively. The antibacterial and antioxidant activities of the extracts were investigated. The methanolic crude extract exhibited resistance towards both bacteria tested; Bacillus subtilisand Escherichia coli, thus suggesting its antibacterial activity.

  1. Abdillah, S., Tambunan, R. M., Farida, Y., Sandhiutami, N. M. D., and Dewi, R. M. (2015). Phytochemical screening and antimalarial activity of some plants traditionally used in Indonesia. Asian Pac. J. Trop. Dis. 5(6), 454-457.
  2. Ajayi, I. A., Oderinde, R. A., Ogunkoya, B. O., Egunyomi, A., and Taiwo, V. O. (2007). Chemical analysis and preliminary toxicological evaluation of Garciniamangostana seeds and seed oil. Food Chem. 101(3), 999-1004.
  3. Amran, A.A., Zaiton, Z., Faizah, O., and Morat, P. (2009). Effects of Garciniaatroviridis on serum profiles and atherosclerotic lesions in the aorta of guinea pigs fed a high cholesterol diet. Singapore Med. J.50(3), 295.
  4. Burkill, I. H. (1966). A Dictionary of the Economic Products of the Malay Peninsula. Crown Agent, London.
  5. Doughari, J. H. (2012). Phytochemicals: Extraction methods, basic structures and mode of action as potential chemotherapeutic agents. InTech.
  6. Downs, B.W., Bagchi, M., Subbaraju, G.V., Shara, M.A., Preuss, H.G., and Bagchi, D. (2005). Bioefficacy of a novel calcium?potassium salt of (−)-hydroxycitric acid. Mutat. Res./Fundamental and Molecular Mechanisms of Mutagenesis. 579(1), 149-162.
  7. Grundy, S. M. (1989). Monounsaturated fatty acids and cholesterol metabolism: Implications for dietary recommendations. Nutr.119(4), 529-533.
  8. Harborne, J. B. (1984). Methods of plant analysis. Springer, Dordrecht.
  9. Irulandi, K., Geetha, S., Tamilselvan, A., and Mehalingam, P. (2016). GC-MS analysis and phytochemical studies of methanolic fruits extract of Garciniacambogia Ex Boerl and Ziziphustrinervia Roth. J. Adv. Res.1(3), 90-95.
  10. Jadid, N., Hidayati, D., Hartanti, S. R., Arraniry, B. A., Rachman, R. Y., &Wikanta, W. (2017). Antioxidant activities of different solvent extracts of Piper retrofractum using DPPH assay. In?AIP Conference Proceedings, Vol. 1854, No.1. AIP Publishing.
  11. Mahadkar, S., Valvi, S., and Jadhav, V. 920130. Gas chromatography-mass spectroscopic (GCMS) analysis of some bioactive compounds from five medicinally relevant wild edible plants. Asian J. Pharm. Clin. Res. 6(1), 136-139.
  12. Manohar S. H., Naik, P. M., Patil, L. M., Karikatti, S. I., and Murthy, H. N. (2014). The chemical composition of Garciniaxanthochymus seeds, seed oil, and evaluation of its antimicrobial and antioxidant activity. Herbs Spices Med. Plants.?20(2), 148-155.
  13. MacKeen, M.M., Ali, A.M., Lajis, N.H., Kawazu, K., Hassan Z., Mohamed H., Mohidin A., Lim Y.M., and Mariam, S. (2000). Antimicrobial, antioxidant, antitumor-promoting and cytotoxic activities of different plant part extracts of Garciniaatroviridis ex T. Anders. ?J. Ethnopharmacol.72(3), 395-402.
  14. Meechai, I., Phupong, W., Chunglok, W., and Meepowpan, P. (2015). GC-MS Analysis of Phytoconstituents in 12 Ma-dan Extracts (Garciniaschomburgkiana). WJST. 13(11), 907-912.
  15. Meechai, I., Phupong, W., Chunglok, W., and Meepowpan, P. (2016). Antioxidant Properties and Phytochemical Contents of Garciniaschomburgkiana JAPS. 6(06), 102-107.
  16. Nursakinah, I., Zulkhairi, H.A., Norhafizah, M., Hasnah, B., ZamreeMd, S., Farrah Shafeera, I., Razif, D., and HamzahFansuri, H. (2012). Nutritional Content and in vitro Antioxidant Potential of Garciniaatroviridis (Asamgelugor) Leaf and Fruits. Malays. J. Nutr. 18(3), 363-71.
  17. Phongpaichit, S., Nikom, J., Rungjindamai, N., Sakayaroj, J., Hutadilok-Towatana, N., Rukachaisirikul, V., &Kirtikara, K. (2007). Biological activities of extracts from endophytic fungi isolated from GarciniaFEMS Immunology & Medical Microbiology,?51(3), 517-525.
  18. Rahmayanti, F., Suniarti, D. F., Subita, G. P., Wimardhani, Y. S., and Masud, Z. A. (2017). Phytochemical Compounds of GarciniaMangostana-Linn Pericarp Fractions from Ethanolic Extract. J. Int. Dent. Med. Res. 10(1).
  19. Roongpisuthipong, C., Kantawan, R., and Roongpisuthipong, W. (2007). Reduction of adipose tissue and body weight: Effect of water soluble calcium hydroxycitrate in Garciniaatroviridis on the short-term treatment of obese women in Thailand. Asia?Pac. J. Clin. Nutr.16(1), 25.
  20. Saeidnia, S., and Abdollahi, M. (2013). Are medicinal plants polluted with phthalates? DARU J. Pharm. Sci. 21(1), 43.
  21. Sakulpanich, A., &Gritsanapan, W. (2009). Determination of anthraquinone glycoside content in Cassia fistula leaf extracts for alternative source of laxative drug.?International journal of biomedical and pharmaceutical sciences,?3(1), 42-45.
  22. Sutar, R.L., Mane, S.P., and Ghosh, J.S. Antimicrobial activity of extracts of dried Kokum (Garciniaindica C). (2012). Int. Food. Res. J.19(3), 1207-1210.
  23. Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria. 191-289.
  24. Tan, W.N., Wong, K.C., Khairuddean, M., Eldeen, I.M., Asmawi, M., and Sulaiman, B. 2013. Volatile constituents of the fruit of Garciniaatroviridis and their antibacterial and anti‐inflammatory activities. J. 28(1), 2-9.
  25. Tisdale, E.J., Kochman, D.A., and Theodorakis, E.A. (2003). Total synthesis of atroviridin. Tetrahedron Lett.44(16), 3281?3284.
  26. Trease, G. E., and Evans, M. C. (1989). Text book of Pharmacognosy 13th Edition BailiereTindall, London, Toronto. Tokyo. 176-180.
  27. Wanxi, P. E. N. G., Shengbo, G. E., Dongli, L. I., Bo, M. O., Daochun, Q., and Ohkoshi, M. (2014). Molecular basis of antibacterial activities in extracts of Eucommiaulmoides PJPS. 27, 6.

[Nur Salsabila Ahmad Roslan, Seema Zareen, Normaiza Zamri and Muhammad Nadeem Akhtar. (2019); PRELIMINARY PHYTOCHEMICAL SCREENING, GC-MS PROFILING AND IN VITRO EVALUATION OF BIOLOGICAL ACTIVITIES OF GARCINIA ATROVIRIDIS ROOT EXTRACTS Int. J. of Adv. Res. (1). 53-61] (ISSN 2320-5407). www.journalijar.com

Muhammad Nadeem Akhtar
Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Malaysia


Article DOI: 10.21474/IJAR01/8295       DOI URL: http://dx.doi.org/10.21474/IJAR01/8295

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