01Mar 2019

POTENTIAL EFFECT OF PROPOLIS AS AN INTRACANAL MEDICAMENT ON ROOT CANAL DISINFECTION AND DENTIN MICROHARDNESS: (AN IN VITRO STUDY).

  • Masters of science, Misr International University, Cairo, Egypt.
  • Lecturer of Endodontics, Misr International University.
  • Professor of Endodontics, Misr International Universty.
Crossref Cited-by Linking logo
  • Abstract
  • References
  • Cite This Article as
  • Corresponding Author

The study aimed to evaluate the potential effect of three intracanal medicaments namely propolis, Calcium hydroxide and Chlorhexidine gel on the root canal disinfection. Materials and method: Sixty-Four samples were chosen for this study. They were divided into four groups, group I received no medication, group II received propolis, group III received Calcium Hydroxide and group IV received Chlorhexidine. The four groups were subdivided into two subgroups A and B, with two-time intervals 1 and 2 weeks. For bacterial evaluation the samples were inoculated with Enterococcus Faecalis. Paper points were placed inside infected root canals and placed in a test tube for CFU count. Results: Results showed that propolis had an intermediate effect on Enterococcus faecalis due to the presence of phenolic compounds in propolis which can eliminated bacteria inside root canal. Calcium hydroxide showed an intermediate effect against bacteria due to its high alkalinity that also can eliminate bacteria. Chlorhexidine showed the highest effect against bacteria due to its ability to stay active inside canal for a long time (substantivity). These results were similar for the 2 time periods. Conclusion: Both Propolis and Ca(OH)2 showed moderate action on intracanal microbes over the two time periods and CHX can be regarded as the best intracanal medicaments regarding its action on intracanal microbes.

  1. Kandaswamy D, Venkateshbabu N, Gogulnath D, Kindo A J. Dentinal tubule disinfection with 2% Chlorhexidine gel, propolis, morindacitrifolia juice, 2% povidone iodine, and calcium hydroxide. IntEndod J. 2010;43(5):419-23.
  2. Borzini L, Cond? R, De Dominicis P, Casaglia A, Cerroni L. Root Canal Irrigation: Chemical Agents and Plant Extracts AgainstEnterococcus faecalis.?Open Dent J. 2016;10:692-703.
  3. Mohammadi Z. Chlorhexidinegluconate, its properties and applications in endodontics.?IntEndod J. 2008;2(4),113-25.
  4. Jahromi MZ, Toubayani H, Rezaei M. Propolis: A new Alternative for Root Canal Disinfection. Iran Endod J. 2012;7:127-33.
  5. Mohammadi Z, Shalavi S, Yazdizadeh M. Antimicrobial Activity of Calcium Hydroxide in Endodontics: A Review.?Chonnam Med J. 2012;48:133-140
  6. Jahromi MZ, Ranjbarian P, Shiravi S. Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts. J Dent Res Dent Clin Dent Prospects. 2014;8:130-133.
  7. Bhandari S, Ashwini TS, Patil CR. An in Vitro Evaluation of Antimicrobial Efficacy of 2% Chlorhexidine Gel, Propolis and Calcium Hydroxide Against Enterococcus faecalis in Human Root Dentin. J ClinDiagn Res. 2014;8:60-63.
  8. Parolia A, Thomas MS, Kundabala M, Mohan M. Propolis and its potential uses in oral health. Int J Med Med Sci. 2010;2:210-15.
  9. Martos MV, Navajas YR, Lopez JF, Alvarez JAP. Functional Properties of Honey, Propolis, and Royal Jelly. J Food Sci. 2008;73(9).
  10. Rezende GPSR, Costa LRRS, Pimenta FC, Baroni DA. In Vitro Antimicrobial Activity of Endodontic Pastes with Propolis Extracts and Calcium Hydroxide: A Preliminary Study. Braz Dent J: 2008;19(4):301-305.
  11. Parolia A, Kundabala M, Rao N, Acharya S, Agrawal P, Mohan M, et al. A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal. Aust Dent J. 2010;55(1):59-64.
  12. Pimenta HC, Violante IMP, Musis CR, Borges AH, Aranha AMF. In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis. Braz Oral Res. 2015;29(1):1-6.
  13. Akca AE, Akca G, Topcu FT, Macit E, Pikdoken L, Ozgen IS. The Comparative Evaluation of the Antimicrobial Effect of Propolis with Chlorhexidine against Oral Pathogens: An in Vitro Study. BioMed Research International. 2016;1-8.
  14. Gomes B, Vianna M, Zaia A, Almeida J, Flho F, Ferraz C. Chlorhexidine in Endodontics. Braz Dent J. 2013; 24(2):89-102.
  15. Pavaskar R, Ataide IN, Chalakkal P, Pinto MJ, Fernandes KS, Keny RV, Kamatb A. An In Vitro Study Comparing the Intracanal Effectiveness of Calcium Hydroxide? and Linezolid-based Medicaments against Enterococcus faecalis. J Endod. 2012;38(1):95-100.
  16. Lima RKP, Guerreiro-Tanomaru JM, Faria-Ju ́nior NB, Tanomaru-Filho M. Effectiveness of calcium hydroxide- based intracanal medicaments against Enterococcus faecalis. IntEndod J. 2012;45(4): 311?316.
  17. Tabrizizadeh M, rasti M, Ayatollahi F, Mossedegh MH, Zandi H, Dehghan F, Mousavi Z. Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment. Iran Endod J. 2015;10(3):184-187.
  18. Wang CS, Arnold RR, Trope M, Teixeira FB. Clinical Efficiency of 2% Chlorhexidine Gel in Reducing Intracanal Bacteria. J Endod. 2007;33(11):1283-89.
  19. Paquette L, Legner M, Fillery ED, Friedman S. Antibacterial Efficacy of ChlorhexidineGluconateIntracanal Medication in Vivo. J Endod. 2007;35(7):788-95.
  20. Ballal V, Kundabala M, Acharya S, Ballal M. Antimicrobial action of calcium hydroxide, chlorhexidine and their combination on endodontic pathogens. Aust Dent J. 2007;52(2):118?21.
  21. Evans M, Baumgartner J, Khemaleelakul S, Xia T. Efficacy of Calcium Hydroxide: Chlorhexidine Paste as an Intracanal Medication in Bovine Dentin. J Endod. 2003;29(5):338?9.
  22. Gomes BPFA, Souza SFC, Ferraz CCR, Teixeira FB, Zaia AA, Valdrighi L, et al. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro. IntEndod J. 2003;36(4):267?75.
  23. Almyroudi A, Mackenzie D, Mchugh S, Saunders W. The Effectiveness of Various Disinfectants Used as Endodontic Intracanal Medications: An In Vitro Study. J Endod. 2002;28(3):163?7.
  24. Stuart C, Schwartz S, Beeson T, Owatz C. Enterococcus faecalis: Its Role in Root Canal Treatment Failure and Current Concepts in Retreatment. J Endod. 2006;32(2):93-98.
  25. Gijo J, Kumar PK, Gopal SS, Kumari S, Reddy BK. Enterococcus faecalis, a nightmare to endodontist: A systematic review. Afr J Microbiol Res. 2015;9(13):898-908.
  26. Bhardwaj SB. Role of Enterococci faecalis in failure of Endodontic treatment.Int J CurrMicrobiol App Sci. 2013;2(8):272-77
  27. Mallic R, Mohanty S, Behera S, Sarangi P, Nanda S, Satapathy SK. Enterococcus faecalis: A resistant microbe in endodontics.Int J Contemp Dent Med Rev. 2014;1-2.
  28. Hazan R, Que Y, Maura D, Rahme LG. A method for high throughput determination of viable bacteria cell counts in 96-well plates.?BMC Microbiol. 2012;12(1):1-7.
  29. Bapat P, Nandy SK, Wangikar P, Venkatesh KV: Quantification of metabolically active biomass using Methylene Blue dye Reduction Test (MBRT): measurement of CFU in about 200 s. J Microbiol Methods. 2006;65:107?16.
  30. Miller JH, Determination of viable cell counts: bacterial growth curves. In Experiments in Molecular Genetics. Edited by Miller JH. New York: Cold Spring Harbor. 1972;31?36.

[Hossam Eldin Ahmed Shaheen, Amr Ahmed Bayoumi and Hossam Mohamed Essam Eldin Tawfik. (2019); POTENTIAL EFFECT OF PROPOLIS AS AN INTRACANAL MEDICAMENT ON ROOT CANAL DISINFECTION AND DENTIN MICROHARDNESS: (AN IN VITRO STUDY). Int. J. of Adv. Res. 7 (Mar). 163-170] (ISSN 2320-5407). www.journalijar.com

Hossam Eldin Ahmed Shaheen
Masters of science, Misr International University, Cairo, Egypt.

DOI:

Article DOI: 10.21474/IJAR01/8616      
DOI URL: http://dx.doi.org/10.21474/IJAR01/8616

Download Full Paper

Download PDF No. of Downloads: 49 | No. of Views: 152