Vol. 8 (02) pp. 775-780 DOI: 10.21474/IJAR01/10508

COMPARATIVE ANALYSIS OF ENAMEL MINERAL PROFILE USING VARIOUS REMINERALIZING AGENTS WITH CONFOCAL LASER RAMAN SPECTROSCOPY - AN INVITROSTUDY

  • Department of Restorative Dental Sciences, Mustaqbal University, Buraidha, Saudi Arabia.
  • Department of Oral and Maxillofacial rehabilitation, Mustaqbal University, Buraidha, Saudi Arabia.
  • General Dental Practitioner, Buraidha, Saudi Arabia.
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Abstract

Aim: To evaluate quantitative changes in enamel mineral profile using CPP-ACP, ACP and Bio-active dentine with confocal laser raman spectroscopy. Materials and Methods: Totally, 25extracted intact pre-molar teeth were collected as specimens. Then the specimens were sectioned at CEJ .The sectioned teeth were evaluated for mineral content using Confocal Laser Raman Spectroscopy (Group ?I).The specimens were placed in demineralizing solution for 4 days and were evaluated for mineral content using Confocal Laser Raman Spectroscopy (Group II).The teeth were then divided into other three groups (6 teeth each) and treated with MI Varnish (Group III) , Enamel Pro Varnish (Group IV), and Bio-active Glass mixed with pro-flouride varnish (Group V) and then dipped in artificial sliva for 5 days, remaining 6 teeth were dipped only in artificial sliva (Group VI) following which the teeth surfaces were studied using Raman spectroscopy to assess the remineralization potential of the three dentifrices. The data were recorded and analyzed statistically. Results: Raman spectroscopic analysis revealed better remineralization with Bio-active Glass mixed with pro-flouride varnish, from the groups treated with the MI Varnishand the Enamel Pro Varnish Conclusion: It can be concluded that the demineralized samples of teeth treated with Bio-active Glass mixed with pro-flouride varnish, was most effective in remineralization of early enamel caries at surface level when analyzed using Raman spectroscopy.

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References

  1. Reynolds EC. Casein phosphopeptide-amorphous calcium phosphate: the scientific evidence. Adv Dent Res 2009 Aug; 21(1):25-29.
  2. Cury JA, Tenuta LMA. Enamel remineralization: controlling the caries disease or treating early caries lesions? Braz Oral Res 2009; 23 (Spec Iss 1): 23-30.
  3. orrado A, Valiente M, Zhang W, Li Y, Munoz C A. Remineralization potential of a new toothpaste formulation: an in vitro study. J Contemp Dent Pract 2004 Feb;5(1):18-30.
  4. Burwell AK, LitkowskiLJ,???????? Greenspan???????????? ???????? Calcium sodium?? phosphosilicate (NovaMin): remineralization potential. Adv Dent Res 2009 Jul;21(1):35-39.
  5. Featherstone JD.Remineralization, the natural caries repair process?the need for new approaches. Adv Dent Res 2009 Jul;21(1):4-7.
  6. Pfarrer AM, Karlinsey RL. Challenges of implementing new remineralization technologies. Adv Dent Res 2009 Jul;21(1):79-82.
  7. Hench LL, West JK. Biological application of bioactive. Life Chem Rep 1996;13:187-91
  8. Madan N, Madan N, Sharma V, Pardal D, Madan N. Tooth remineralization using bio-active glass - A novel approach. J AcadAdv Dent Res 2011; 2: 45-50.
  9. Reminerization Effect of Bioactive Glass on Enamel Caries-like Lesions. Southeast Asian J Trop Med Public Health. Vol 48 No. 5 September 2017
  10. Walsh LJ. Contemporary technologies for remineralization therapies: a review. Int Dent SA 2009 Jan;4:34-46.
  11. RRamakrishnaiah, G.Rehman , A.A.Alkhulufi ,etal. Applications of Raman Spectroscopy in Dentistry: Analysis of Tooth Structure.J Applied spectroscopyReview2015: Vol 50: issue 4 : 332 -350
  1. Hassanein OE, El-Brolossy TA. An investigation about the reminerlization potential of bioactive glass on artificially carious enamel and dentin using Raman spectroscopy. Egypt J Solids 2006; 29: 69-80.
  2. Tisson V Job, Girish T Narayanaetal .Remineralization Potential of Three Different Dentifrices. The Journal of Contemporary Dental Practice, April 2018;19(4):420-425
  3. Mehta AB, Kumari V, Jose R, Izadikhah V. Remineralization potential of bioactive glass and casein phosphopeptide-amorphous calcium phosphate on initial carious lesion: an in-vitro pH-cycling study. J Conserv Dent 2014; 17: 3-7.

How to Cite This Article

Mudit Uppal, Gurleen Arora and Tuqa Hamed (2020); COMPARATIVE ANALYSIS OF ENAMEL MINERAL PROFILE USING VARIOUS REMINERALIZING AGENTS WITH CONFOCAL LASER RAMAN SPECTROSCOPY - AN INVITROSTUDY, Int. J. of Adv. Res., 8 (02), 775-780, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/10508

Corresponding Author

Dr. Mudit Uppal
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