Design, Screening and Microbial Synthesis of Bio-polymers of Poly-Hydroxy-Butyrate (PHB) from Low Cost Carbon Sources
Abstract: The environmental pollution by petro based plastics a cause of concern, which are non biodegradable. Hence biodegradable, biologically synthesized polymers with similar properties of conventional plastic are sought. Poly-?-hydroxy-butyrate (PHB) is a member of a family of polyhydroxyalkonates synthesized by numerous bacteria as an intracellular carbon and energy storage compound under nutrient-limiting conditions with excess carbon. Among microbially formed Poly HydroxyAlkanotes (PHA) is biodegradable, water soluble, non-toxic, bio-compatible, piezoelectric, thermoplastic and elastomeric. It has wide applications in different areas such as medicines, long term dosage of drugs, cosmetic world, cosmetic containers, shampoo bottles, insecticides, fertilizers, packing materials, disposable items such as razors, diapers, feminine hygienic products etc. Media formulations were designed with different compositions of Molases/Glycerol/Glucose for enhancement of bio-polymer synthesis. Microbes were screened for PHB production by Sudan black staining of the colonies. Cells were broken with hypochlorite and PHB extracted into chloroform and its content was analyzed by Spectrophotometer at 235nm using crotonic acid as standard. Ten isolates out of 16 could produce PHB. Isolate #10 gave 38.46% PHB when grown on bio-diesel derived glycerol whereas isolate # 3 gave only 8% PHB when grown on molasses.
[Lingayya Hiremath, Narendra Kumar S, Ravishankar H.N, Swathi Angadi and Sukanya P. (2015); Design, Screening and Microbial Synthesis of Bio-polymers of Poly-Hydroxy-Butyrate (PHB) from Low Cost Carbon Sources Int. J. of Adv. Res. 3 (2). 0] (ISSN 2320-5407). www.journalijar.com
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