The influence of Leukocytospermia and Teratozoospermia in IUI outcome

Background: Male urogenital tract infection is one of the most important causes of male infertility worldwide. The presence of leukocytes in the semen has been associated with poor semen quality and positive correlation between leukocytospermia and abnormal sperm morphology. Objective: This investigation will be analyzed the outcome of the intra uterine insemination (IUI) cycles in which the male partner exhibited leukocytospermia and/or teratozoospermia. Subjects, Materials and methods: This study includes seventy men their ages between 20- 45 years old who attended to High Institute of Infertility Diagnosis and Assisted Reproductive Technology / AL-Nahrain University and Al-Imamein Kadhymia Medical City at Um Al- Banein Center .This prospective study was accomplished through the period from November 2014 till March 2015. Results: The study found that sperm concentration in the forth groups were significantly (P<0.01) lower after in vitro sperm activation(ISA) compared with before ISA. The active Sperm motility a+b and morphologically sperm normality were reached the highly significant (p<0.01) increase after ISA compared with before ISA. The number of round cells after ISA was a significantly (P<0.01) decreased compared to before ISA, the mean of leukocytes by Endtz test method after ISA was significantly (P<0.01) reduced compared with before ISA. The total pregnancy rate after ISA was (12.8%) per cycle after in vitro activation technique and IUI, the pregnancy rates following IUI in normozoospermic group (20%) ,leukocytospermic group (13.3%), teratozospermic group(6.6%) and leukocytospermic- teratozospermic group (6.6%). Conclusions: The pregnant rate after IUI in leukocytospermic and teratozoospermic couples was low compared to normozoospermic couples. Thus the infertile men with abnormal sperm form and high number of leukocytes are recommended to ensure the sperm normality following in vitro activation techniques by Kruger criteria and peroxidase (ENDTZ) test with cytogenetic study before intra uterine insemination.