The outer membrane proteins profile of Salmonella enterica serotypes Enteritidis, Muenster, Florian, Omuna and Noya and their dendrogram analysis

Non-typhoidal Salmonella is increasingly recognized as an important pathogen associated with gastroenteritis. However, there is limited data specifically describing the Outer membrane proteins (OMPs), a distinguishing feature of Gram negative bacteria located at host–bacterial interface and are important for virulence, host immune responses and as targets for drug therapy. The present study is based on molecular characterization of four Salmonella serotypes representing three serologically different serogroups (Muenster, Florian, Omuna and Noya) and their relatedness to a S. Enteritidis reference strain. The OMPs profiling of the S. enterica serotypes were differentiated by SDS-PAGE using a Triton X-100-lysozyme-EDTA extraction method. Eighteen protein bands could be resolved ranging in size from 61.0 kDa to 14.2 kDa. The Salmonella serotypes used in this study were un-related and could be differentiated depending on their OMPs. The Dice index similarity coefficients between serotypes ranged from 52% to 85.7%. In conclusion, the identification of the low molecular weight fractions of Salmonella OMP in our study need to be correlated with the incidence of Salmonella induced reactive arthritis (ReA)/undifferentiated spondyloarthropathy (uSpA) in Egypt. This will help in identifying the T cell immunodominant antigens which can be used for vaccine or for developing a diagnostic test for ReA/uSpA.