COMPARATIVE STUDIES OF L-LACTICACID PRODUCTION FROM GROUND NUT SHELL AND SUGARCANE MOLASSES BY MUTANT LACTOBACILLUS DELBRUECKIINCIM2025 U-25 STRAIN.

The lactic acid production from groundnut shells is economical since the raw material is cheaply available source even though the yield of lactic acid is about 23g/lit. This would be a potential source for producing the lactic acid. However the lactic acid from other sources like sugarcane molasses is tried to enhance the yield. It is observed that lactic acid yield is 23.5g/lit from the sugar concentration of 20g/lit. Which is more than the strains without mutations, which is about 20g/lit. The mutant cells were tested for its stability on 0.6% Allyl alcohol, which was a selection agent. Mutants were generated by exposing to UV radiations, with different exposure times and stability is tested. The results were encouraging and the lactic acid produced was about 30 g/l from groundnut shells and 23.5g/lit from sugarcane molasses at temperature of 400C. The pH optimum was found to be about 5.2 and inoculum size was found to be 3 ml. The present comparative studies were focused on the concentration of lactic acid from groundnut shell and sugarcane molasses by mutant strain U-20.