EXTRACTION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE ENZYME FROM ISOLATED BACTERIUM BACILLUS SUBTILIS AND OPTIMIZATION OF FEW CULTURE CONDITIONS
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The aim of this study is to optimize the environmental conditions during fermentation, developing an industrial media formulation and evaluating the characteristic properties of the crude alkaline protease enzyme produced from Bacillus sp. isolated from natural habitat. Bacteria of genus Bacillus are active producers of extracellular proteases. Bacillus subtilis was allowed to grow in broth culture for purpose of inducing protease enzyme. Proteases are enzymes that hydrolyse proteins via the addition of water across peptide bonds and catalyses peptide synthesis in organic solvents and in solvents with low water content. The purification was carried by applying successively dialysis, DEAE-cellulose ion exchange chromatography to the supernatant. In this study the optimum temperature (40ºC), optimum pH (7.0), optimum incubation period (42 h), salt concentration (0.05gm) and sugar source (sucrose) were determined. Molecular weight of the obtained enzyme was investigated by SDS-PAGE. Molecular weight of the protease was determined, and it was found that the weight of enzyme was 63 kDa. Data emphasized the possibility of extraction and partial purification of protease enzyme from Bacillus subtilis for industrial scale applications.
[A.V.N. Tejaswini, Sanjeev Ranjan and V. Sridevi (2014); EXTRACTION, PARTIAL PURIFICATION AND CHARACTERIZATION OF PROTEASE ENZYME FROM ISOLATED BACTERIUM BACILLUS SUBTILIS AND OPTIMIZATION OF FEW CULTURE CONDITIONS Int. J. of Adv. Res. 2 (Jan). 0] (ISSN 2320-5407). www.journalijar.com
