PRODUCTION OF L-GLUTAMINASE FROM MARINE ECOSYSTEMS AND OPTIMAL CONDITIONS FOR MAXIMAL PRODUCTION BY ACTINOMYCETES

L-glutaminase is an enzyme produced by various microorganisms which are currently used for the treatment of leukemia, as flavour enhancer and also as enzyme sensors. Actinomycetes have been recognize as rich source of glutamaminase production enzyme. Even though L-glutaminase activity was reported in various microorganisms, L-glutaminase from actinomycetes in general and marine actinomycete in particular is very scanty. Attempts are now being made to replace enzymes which traditionally have been isolated from animal tissues and plants with enzymes from microorganisms. Solid state fermentation is economically advantageous. Actinomycetes from terrestrial and marine ecosystems have long been recognized either as organism of academic curiosity and organism of antibiotic producers and marine Actinomycetes are effective in enzyme production. Among different substrates Wheat bran was the best substrate for induction of L-glutaminase.The strain of Actinomycetes was used for glutamic acid production under optimum growth conditions. L-glutaminase was assayed by direct Nesslerization measured at 480 nm using a spectrophotometer. Actinomycetes was used for enhancing glutamic acid.The strain gave maximum production at pH 7, temperature 30°C, time 96hours and salinity 3.5%.