PREVALENCE OF HUMAN PAPILLOMA VIRUS IN CERVICAL CELLS AMONG PATIENTS WITH ABNORMAL PAP SMEARS.
- Ph.D. student Genetic Engineering and Biotechnology Institute for Postgraduate Studies / University of Baghdad.
- Asst.Prof.Genetic Engineering and Biotechnology Institute for Postgraduate Studies / University of Baghdad.
- Prof.Director of National Center for Cancer Research/ University of Baghdad.
- Prof.College of medicine / University of Baghdad.
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Abstract
Human papillomavirus (HPV) is the most common viral infection of the reproductive tract. Cervical cancer is by far the most common HPV-related disease. Nearly more cases of cervical cancer can be attributable to HPV infection.This study designed foe molecular detections and genotyping of human papilloma virus (HPV) by designing methods - Endpoint real time PCR for HPV detections and genotyping of HPV 16 and 18.
The study includes 90 Pap smear samples of women suffering from abnormal Pap smears and 10 healthy women as a control , were collected from the Baghdad hospital ( Medical City), Oncology hospital and Al- Amal hospital (in Baghdad), during the period from April, 2015 to April, 2016. The age of women was ranged from 16 to 60 years. The molecular detection method (Designed PCR) in our study was showed that the 35% of women have HPV infections .
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References
- Tarney, C. and Han, J. (2014). Postcoital bleeding: a review on etiology, diagnosis, and management. Obstetrics and gynecology international . PMID : 192087. 25045355.
- Al-Alwan, N. (1995). The Fate of Mild Cervical Dysplasia: a long-term cytologic follow-up study of 252 patients. Fac. Med. Baghdad Univ.; 37: 237-244).
- Al-Alwan, N. (2001). Colposcopy, Cervical Cytology and Human Papillomavirus Detection as Screening tools for Cervical Cancer. Eastern Mediterranean Health Journal, WHO, EMRO, 7 (1/2): 100-105.
- Kufe, D. (2009). Holland-Frei cancer medicine. (8th ed.). New York: McGraw-Hill Medical. p. 1299. ISBN 9781607950141.
- Michael,F. ; Charlotte, C. ; Sun,L. Schover, M. ; Munsell, A. ; Taylor, W. ; Patricia E. ; Therese, B. ; Charles, F. ; Levenback,C. ; David, M. and Diane,C. (2005). Quality of Life and Sexual Functioning in Cervical Cancer Survivors. Journal of Clinical Oncology. 23 , No. 30 :7428-7436.
- Moyer,V. ; Preventive, U. and Task, F. (2012). Screening for cervical cancer: U.S. Preventive Services Task Force recommendation statement. Annals of internal medicine 156 (12): 880?91, W312.
- Brestovac, B. ; Wong, M. ; Costantino, P. and Groth, D. (2014). A rapid DNA extraction method suitable for human papillomavirus detection. J Med Virol. 653-7. doi: 10.1002/jmv.23882.
- Sambrook, J. (1989). Molecular? Cloning: A Laboratory manual. Second Edition. (Plainview, New York: Cold Spring Harbor Laboratory Press).
- Venceslau1 E.; Bezerra, M.; Lopes, A.; Souza, ?.; Onofre, A. ; De Melo C.; Jeraldo ,V. and Onofre, F.(2014). HPV detection using primers MY09/MY11 and GP5+/GP6+ in patients with cytologic and/or colposcopic changes. J Bras Patol Med Lab, v. 50, n. 4, p. 280-285.
- [9]. Richart, R. (1972). Proceedings: An assessment of the biology of cervical intraepithelial neoplasia. Proc Natl Cancer Conf. 7:219?22.
- Mark, S. and Nicolas, W. (2013). Human papillomavirus (HPV) infection and the multi-stage carcinogenesis of cervical cancer. Cancer Epidemiol Biomarkers Prev. 22(4): 553?560. doi:10.1158/1055-9965.EPI-12-1406.
- Emanuella, M.; Mauro, M.; Anna, C.; ?rick, V.; Alexandre, S.; Claudia, M.; V. ; Fabiana, B.(2014). HPV detection using primers MY09/MY11 and GP5+/GP6+ in patients with cytologic and/or colposcopic changes. J Bras Patol Med Lab, v. 50, n. 4, p. 280-285.
- Demathe, A. (2010). Compara??o entre dois m?todos de detec??o de DNAde papilomav?rus humano em carcinoma epidermoide de l?bio. J BrasPatol Med Lab, v. 46, n. 2, p. 85-90.
- Julie, L. ; Kirstin, E. and Nick, S. (2009). Real-Time PCR Current Technology and Applications. Caister Academic Press. ISBN 978-1-904455-39-4.
How to Cite This Article
Mohammed Ibraheem Mezaal, Ismail Husein Aziz, Maad Mahdi Shalal and Nada Alwan. (2017); PREVALENCE OF HUMAN PAPILLOMA VIRUS IN CERVICAL CELLS AMONG PATIENTS WITH ABNORMAL PAP SMEARS., Int. J. of Adv. Res., 5 (12), 1393-1400, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/6099
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