Comparative dendrogram analysis of OMPs of Salmonella enterica serotype Enteritidis with Typhimurium, Braendurup and Lomita isolated from pigeons

Outer membrane proteins (OMPs) is a distinguishing feature of Gram negative bacteria located at host–bacterial interface and are important for virulence, host immune responses and as targets for drug therapy. In the present study, Omps profiling of three Salmonella enterica serotypes (Typhimurium, Braendurup and Lomita) obtained from pigeons and implicated in human salmonellosis were differentiated by SDS-PAGE using a Triton X-100-lysozyme-EDTA extraction method. More than 21 protein bands could be resolved ranging in size from 61.0 kDa to 7.5 kDa as analyzed using Gel Compar II software of their approximate molecular masses. The Salmonella serotypes used in this study were un-related and could be differentiated depending on their OMPs profiles using SDS-PAGE. Dendrograms were constructed by the unweighted pair-group using the average linkage clustering method. The Dice index similarity coefficients between serotypes ranged from 45% to 100%.